ArticleBMC biotechnology2005
Goat uromodulin promoter directs kidney-specific expression of GFP gene in transgenic mice.
Article in BMC biotechnology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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2 citing papers in PubMed, 6 citations in OpenAlex.
- Substantially improved pharmacokinetics of recombinant human butyrylcholinesterase by fusion to human serum albumin.BMC biotechnology · 2008Article
- UT-A urea transporter promoter, UT-Aalpha, targets principal cells of the renal inner medullary collecting duct.American journal of physiology. Renal physiology · 2006Article
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6 authors at 3 institutions in 2 countries.
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Abstract
backgroundUromodulin is the most abundant protein found in the urine of mammals. In an effort to utilize the uromodulin promoter in order to target recombinant proteins in the urine of transgenic animals we have cloned a goat uromodulin gene promoter fragment (GUM promoter) and used it to drive expression of GFP in the kidney of transgenic mice.
resultsThe GUM-GFP cassette was constructed and transgenic mice were generated in order to study the promoter's tissue specificity, the GFP kidney specific expression and its subcellular distribution. Tissues collected from three GUM-GFP transgenic mouse lines, and analyzed for the presence of GFP by Western blotting and fluorescence confirmed that the GUM promoter drove expression of GFP specifically in the kidney. More specifically, by using immuno-histochemistry analysis of kidney sections, we demonstrated that GFP expression was co-localized, with endogenous uromodulin protein, in the epithelial cells of the thick ascending limbs (TAL) of Henle's loop and the early distal convoluted tubule in the kidney.
conclusionThe goat uromodulin promoter is capable of driving recombinant protein expression in the kidney of transgenic mice. The goat promoter fragment cloned may be a useful tool in targeting proteins or oncogenes in the kidney of mammals.
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