ArticlePlant molecular biology2004
Ectopic expression of a soybean phytase in developing seeds of Glycine max to improve phosphorus availability.
Article in Plant molecular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 77 citations in OpenAlex.
- Metabolic Study of Cucumber Seeds and Seedlings in the Light of the New, Controversial Trend of Preventive Use of Systemic Fungicides.International journal of molecular sciences · 2023Article
- Isolation and pan-genome analysis ofFrontiers in microbiology · 2023Article
- Reverse genetic approaches for breeding nutrient-rich and climate-resilient cereal and food legume crops.Heredity · 2022Article
- Molecular Mechanisms and Biochemical Pathways for Micronutrient Acquisition and Storage in Legumes to Support Biofortification for Nutritional Security.Frontiers in plant science · 2021Review
- Network Inference of Transcriptional Regulation in Germinating Low Phytic Acid Soybean Seeds.Frontiers in plant science · 2021Article
- Bioprocess for Production, Characteristics, and Biotechnological Applications of Fungal Phytases.Frontiers in microbiology · 2020Review
- Diverse Enzymes With Industrial Applications in Four Thraustochytrid Genera.Frontiers in microbiology · 2020Article
- Transgenic soybean expressing a thermostable phytase as substitution for feed additive phytase.Scientific reports · 2019Article
- Genetic transformation of tropical maize (3 Biotech · 2019Article
- Development and Evaluation of Low Phytic Acid Soybean by siRNA Triggered Seed Specific Silencing ofFrontiers in plant science · 2018Article
- Heterologous Expression of Secreted Bacterial BPP and HAP Phytases in Plants StimulatesFrontiers in plant science · 2018Article
- Genetically modified phytase crops role in sustainable plant and animal nutrition and ecological development: a review.3 Biotech · 2017Review
- A novel cis-acting element in the GmERF3 promoter contributes to inducible gene expression in soybean and tobacco after wounding.Plant cell reports · 2016Article
- Regulation of phosphorus uptake and utilization: transitioning from current knowledge to practical strategies.Cellular & molecular biology letters · 2016Review
- The intron and 5' distal region of the soybean Gmubi promoter contribute to very high levels of gene expression in transiently and stably transformed tissues.Plant cell reports · 2015Article
- Development of low phytate rice by RNAi mediated seed-specific silencing of inositol 1,3,4,5,6-pentakisphosphate 2-kinase gene (IPK1).PloS one · 2013Article
- Overexpression of phyA and appA genes improves soil organic phosphorus utilisation and seed phytase activity in Brassica napus.PloS one · 2013Article
- Recent advances in soybean transformation and their application to molecular breeding and genomic analysis.Breeding science · 2012Article
- A fluorescent reporter protein containing AtRMR1 domains is targeted to the storage and central vacuoles in Arabidopsis thaliana and tobacco leaf cells.Plant cell reports · 2011Article
- Genetic improvement for phosphorus efficiency in soybean: a radical approach.Annals of botany · 2010Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A transgenic approach was used to alter soybean seed phytate content by expressing a soybean phytase gene (GmPhy) during seed development to degrade accumulating phytic acid (IP6). An expression vector containing the soybean phytase cDNA controlled by the seed-specific beta-conglycinin promoter (alpha'-subunit) was used to transform embryogenic soybean cultures. Plants from four independent transgenic lines were analyzed for transgene integration and seed IP6 levels. The reduction in IP6 levels in transgenic seeds compared to control 'Jack' soybeans ranged from 12.6 to 24.8 as determined by HPLC. A low copy transformant was propagated to the T4 generation and examined in more detail for phytase expression and enzyme activity during seed development. Expression of phytase mRNA and phytase activity increased during seed development, consistent with the use of an embryo-specific promoter. Ectopic phytase expression during seed development offers potential as an effective strategy for reducing phytate content in soybean seed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.