ArticlePlant molecular biology2004
Ammonium transporter genes in Chlamydomonas: the nitrate-specific regulatory gene Nit2 is involved in Amt1;1 expression.
Article in Plant molecular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 84 citations in OpenAlex.
- Client distribution between Chlamydomonas FDX1 and FDX2 in carbon, nitrogen and sulfur assimilation.bioRxiv : the preprint server for biology · 2026Article
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- Identification of the MAPK Cascade and its Relationship with Nitrogen Metabolism in the Green AlgaInternational journal of molecular sciences · 2020Article
- Origin Recognition Complex (ORC) Evolution Is Influenced by Global Gene Duplication/Loss Patterns in Eukaryotic Genomes.Genome biology and evolution · 2020Article
- Transcriptomic analysis reveals recovery strategies in strawberry roots after using a soil amendment in continuous cropping soil.BMC plant biology · 2020Article
- New molecular insights on the response of the green alga Tetraselmis suecica to nitrogen starvation.Scientific reports · 2019Article
- Article
- Characterization of a Mutant Deficient for Ammonium and Nitric Oxide Signalling in the Model System Chlamydomonas reinhardtii.PloS one · 2016Article
- Understanding nitrate assimilation and its regulation in microalgae.Frontiers in plant science · 2015Review
- Nitrogen-Sparing Mechanisms in Chlamydomonas Affect the Transcriptome, the Proteome, and Photosynthetic Metabolism.The Plant cell · 2014Article
- Nitric oxide-triggered remodeling of chloroplast bioenergetics and thylakoid proteins upon nitrogen starvation in Chlamydomonas reinhardtii.The Plant cell · 2014Article
- Combined intracellular nitrate and NIT2 effects on storage carbohydrate metabolism in Chlamydomonas.Journal of experimental botany · 2014Article
- Ammonium and urea transporter inventory of the selaginella and physcomitrella genomes.Frontiers in plant science · 2012Article
- The cloning and characterization of two ammonium transporters in the salt-resistant green alga, Dunaliella viridis.Molecular biology reports · 2011Article
- Synchronization of cytoplasmic and transferred mitochondrial ribosomal protein gene expression in land plants is linked to Telo-box motif enrichment.BMC evolutionary biology · 2011Article
- Algae and humans share a molybdate transporter.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Predicting the physiological role of circadian metabolic regulation in the green alga Chlamydomonas reinhardtii.PloS one · 2011Article
- Changes in transcript abundance in Chlamydomonas reinhardtii following nitrogen deprivation predict diversion of metabolism.Plant physiology · 2010Article
- The mixed lineage nature of nitrogen transport and assimilation in marine eukaryotic phytoplankton: a case study of micromonas.Molecular biology and evolution · 2010Article
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3 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Ammonium transport is a key process in nitrogen metabolism. In the green alga Chlamydomonas, we have characterized molecularly the largest family of ammonium transporters (AMT1) so far described consisting of eight members. CrAmt1 genes have an interesting transcript structure with some very small exons. Differential expression patterns were found for each CrAmt1 gene in response to the nitrogen source by using Real Time PCR. These expression patterns were similar under high and low CO2 atmosphere. CrAmt1;1 expression was characterized in detail. It was repressed in both ammonium and nitrate medium, and strongly expressed in nitrogen-free media. Treatment with a Glutamine synthetase inhibitor released partially repression in ammonium and nitrate suggesting that ammonium and its derivatives participate in the observed repressing effects. By studying CrAmt1;1 expression in mutants deficient at different steps of the nitrate assimilation pathway, it has been shown that nitrate has a double negative effect on this gene expression; one related to its reduction to ammonium, and a second one by itself. This second effect of nitrate was dependent on the functionality of the regulatory gene Nit2, specific for nitrate assimilation. Thus, NIT2 would have a dual role on gene expression: the well-known positive one on nitrate assimilation and a novel negative one on Amt1;1 regulation.
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