ArticlePlant molecular biology2005
Stable expression of a defense-related gene in wheat epidermis under transcriptional control of a novel promoter confers pathogen resistance.
Article in Plant molecular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 98 citations in OpenAlex.
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- CsPrx25, a class III peroxidase in Citrus sinensis, confers resistance to citrus bacterial canker through the maintenance of ROS homeostasis and cell wall lignification.Horticulture research · 2020Article
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- High Resolution Genetic and Physical Mapping of a Major Powdery Mildew Resistance Locus in Barley.Frontiers in plant science · 2019Article
- Isolation and characterization of rubisco small subunit gene promoter from common wheat (Triticum aestivum L.).Plant signaling & behavior · 2015Article
- Plant disease resistance is augmented in uzu barley lines modified in the brassinosteroid receptor BRI1.BMC plant biology · 2014Article
- Discovery of genes affecting resistance of barley to adapted and non-adapted powdery mildew fungi.Genome biology · 2014Article
- Genomic stability and long-term transgene expression in poplar.Transgenic research · 2013Article
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- Allele mining in barley genetic resources reveals genes of race-non-specific powdery mildew resistance.Frontiers in plant science · 2011Article
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- Sugarcane DIRIGENT and O-methyltransferase promoters confer stem-regulated gene expression in diverse monocots.Planta · 2010Article
- Regulation of gene expression by chromosome 5A during cold hardening in wheat.Molecular genetics and genomics : MGG · 2010Article
- Promoters of the barley germin-like GER4 gene cluster enable strong transgene expression in response to pathogen attack.The Plant cell · 2010Article
- A class III peroxidase specifically expressed in pathogen-attacked barley epidermis contributes to basal resistance.Molecular plant pathology · 2008Article
- Tissue-specific expression of a defence-related peroxidase in transgenic wheat potentiates cell death in pathogen-attacked leaf epidermis.Molecular plant pathology · 2008Article
- A set of modular binary vectors for transformation of cereals.Plant physiology · 2007Article
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tissue-specific or regulated expression of transgenes is desirable in order to prevent pleiotropic side effects of putatively harmful transgene products as well as loss of energy resources due to unnecessary accumulation of transgene products. Epidermis-specific expression would be useful for many defense-related genes directed against attack by fungal pathogens that enter the plant body by direct penetration through the epidermis. In an approach to enhance resistance of wheat to the powdery mildew fungus Blumeria graminis f.sp. tritici, a novel epidermis-specific promoter was developed and used for expression of two defense-related genes. A 2.3 kb fragment of the wheat GstA1 promoter in combination with an intron-containing part of the wheat WIR1a gene was found to drive strong and constitutive transient expression in wheat epidermis. This promoter-intron combination was used for overexpression of oxalate oxidase 9f-2.8 and TaPERO peroxidase, two defense-related wheat genes expressed in inner leaf tissues. Expression studies of several transgenic lines by in situ oxalate-oxidase staining, RNA and protein blot analyses, as well as real-time PCR, demonstrated strong and constitutive transgene expression in the shoot epidermis. Transient as well as stable over-expression of the TaPERO peroxidase gene in wheat epidermis under the control of the GstA1i promoter resulted in enhanced resistance against Blumeria graminis f.sp. tritici, whereas oxalate-oxidase overexpression had no effect in either system. The data suggest that the wheat GstA1 promoter in combination with the WIR1a intron is useful for transgenic approaches to fungal disease resistance in cereals.
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