ArticlePlant molecular biology2005
Specific interactions between Dicer-like proteins and HYL1/DRB-family dsRNA-binding proteins in Arabidopsis thaliana.
Article in Plant molecular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 129 papers.
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129 citing papers in PubMed, 290 citations in OpenAlex.
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- Plant microRNA maturation and function.Nature reviews. Molecular cell biology · 2026Review
- Genome-wide identification and characterization ofFrontiers in plant science · 2026Article
- DRB1 and DRB2 Are Required for an Appropriate miRNA-Mediated Molecular Response to Salt Stress inPlants (Basel, Switzerland) · 2025Article
- Identification and characterization of DICER-LIKE genes and their roles in Marchantia polymorpha development and salt stress response.The Plant journal : for cell and molecular biology · 2025Article
- The Use of Arabidopsis thaliana to Characterize the Production and Action Stages of the Plant MicroRNA Pathway.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Clade-wide fungal proteome analysis reveals structure-function conservation in divergent Dicer proteins.Computational and structural biotechnology journal · 2025Article
- Molecular Manipulation of the miR160/Genes · 2024Article
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- Exogenous and endogenous dsRNAs perceived by plant Dicer-like 4 protein in the RNAi-depleted cellular context.Cellular & molecular biology letters · 2023Article
- Phylogenetic analyses of seven protein families refine the evolution of small RNA pathways in green plants.Plant physiology · 2023Article
- SEMI-ROLLED LEAF 10 stabilizes catalase isozyme B to regulate leaf morphology and thermotolerance in rice (Oryza sativa L.).Plant biotechnology journal · 2023Article
- Genome-wide identification, characterization and expression analysis of AGO, DCL, and RDR families in Chenopodium quinoa.Scientific reports · 2023Article
- Turnip crinkle virus-encoded suppressor of RNA silencing interacts with Arabidopsis SGS3 to enhance virus infection.Molecular plant pathology · 2023Article
- microRNA production inFrontiers in plant science · 2023Review
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- RNAi-Based Antiviral Innate Immunity in Plants.Viruses · 2022Review
- Multi-omics data integration reveals link between epigenetic modifications and gene expression in sugar beet (Beta vulgaris subsp. vulgaris) in response to cold.BMC genomics · 2022Article
- Article
69 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proteins that specifically bind double-stranded RNA (dsRNA) are involved in the regulation of cellular signaling events and gene expression, and are characterized by a conserved dsRNA-binding motif (dsRBM). Here we report the biochemical properties of nine such gene products, each containing one or two dsRBMs: four Arabidopsis Dicer-like proteins (DCL1-4), Arabidopsis HYL1 and four of its homologs (DRB2, DRB4, DRB5 and OsDRB1). DCL1, DCL3, HYL1 and the four HYL1 homologs exhibit significant dsRNA-binding activity, indicating that these proteins are involved in RNA metabolism. The dsRBMs from dsRBM-containing proteins (dsRBPs) also function as a protein-protein interaction domain and homo- and heterodimerization are essential for biological functioning of these proteins. We show that DRB4 interacts specifically with DCL4, and HYL1 most strongly interacts with DCL1. These results indicate that each HYL1/DRB family protein interacts with one specific partner among the four Dicer-like proteins. Localization studies using GFP fusion proteins demonstrate that DCL1, DCL4, HYL1 and DRB4 localize in the nucleus, while DRB2 is present in the cytoplasm. Subcellular localizations of HYL1, DRB4, DCL1 and DCL4 further strengthen the notion that HYL1 and DCL1, and DRB4 and DCL4, exist as complexes. The presented data suggest that each member of the HYL1/DRB protein family may individually modulate Dicer function through heterodimerization with a Dicer-like protein in vivo.
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