ArticlePlant molecular biology2005
Transcriptional activation of tobacco E2F is repressed by co-transfection with the retinoblastoma-related protein: cyclin D expression overcomes this repressor activity.
Article in Plant molecular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 50 citations in OpenAlex.
- Sugar signals pedal the cell cycle!Frontiers in plant science · 2024Review
- Engineered gamma radiation phytosensors for environmental monitoring.Plant biotechnology journal · 2023Article
- Hydroxyurea and Caffeine Impact pRb-like Protein-Dependent Chromatin Architecture Profiles in Interphase Cells ofInternational journal of molecular sciences · 2021Article
- Roles of plant retinoblastoma protein: cell cycle and beyond.The EMBO journal · 2020Review
- Lineage- and stage-specific expressedDevelopment (Cambridge, England) · 2018Article
- Cell-size dependent progression of the cell cycle creates homeostasis and flexibility of plant cell size.Nature communications · 2017Article
- The MADS-box XAANTAL1 increases proliferation at the Arabidopsis root stem-cell niche and participates in transition to differentiation by regulating cell-cycle components.Annals of botany · 2016Article
- Low Temperature Affects Stem Cell Maintenance in Brassica oleracea Seedlings.Frontiers in plant science · 2016Article
- Cyclin-Dependent Kinase Regulation of Diurnal Transcription in Chlamydomonas.The Plant cell · 2015Article
- Composition, roles, and regulation of cullin-based ubiquitin e3 ligases.The arabidopsis book · 2014Article
- Arabidopsis E2FA stimulates proliferation and endocycle separately through RBR-bound and RBR-free complexes.The EMBO journal · 2012Article
- Partners in time: EARLY BIRD associates with ZEITLUPE and regulates the speed of the Arabidopsis clock.Plant physiology · 2011Article
- Regulation of the Chlamydomonas cell cycle by a stable, chromatin-associated retinoblastoma tumor suppressor complex.The Plant cell · 2010Article
- Positive regulation of minichromosome maintenance gene expression, DNA replication, and cell transformation by a plant retinoblastoma gene.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Arabidopsis RETINOBLASTOMA-RELATED PROTEIN 1 is involved in G1 phase cell cycle arrest caused by sucrose starvation.Plant molecular biology · 2008Article
- Genomic organization and evolutionary conservation of plant D-type cyclins.Plant physiology · 2007Article
- Induction of differentiation in the shoot apical meristem by transient overexpression of a retinoblastoma-related protein.Plant physiology · 2006Article
- Cell cycle regulated D3-type cyclins form active complexes with plant-specific B-type cyclin-dependent kinase in vitro.Plant molecular biology · 2006Article
- Expression of cell cycle genes in shoot apical meristems.Plant molecular biology · 2006Review
- RBR3, a member of the retinoblastoma-related family from maize, is regulated by the RBR1/E2F pathway.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Evidence is emerging that the E2F family of transcription factors plays an important role in the regulation of gene expression at the G1/S transition in plants. Here, we show that in the tobacco proliferating cell nuclear antigen (PCNA), whose transcript is specifically expressed at G1/S phase, the two E2F binding sites are synergistically responsible for transcriptional activation at G1/S phase in synchronized tobacco BY-2 cells transformed with promoter constructs fused to a reporter gene. In addition, we have isolated the tobacco DP cDNA (NtDP) and showed that significant activation of the reporter gene was observed in transient expression assays by concomitantly transfecting with plasmids expressing NtE2F and NtDP. This transcriptional activation was repressed by co-transfection with a plasmid expressing NtRBR1; in vitro pull-down assays also revealed that NtRBR1 binds directly to NtE2F, thereby potentially blocking the transcriptional activation of NtE2F. Importantly, this repressor activity was cancelled when NtRBR1 was further co-transfected with a plasmid expressing cyclin D but not with cyclin A or cyclin B. These results are discussed with respect to the repression activity of NtRBR1 on the NtE2F/NtDP complex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.