ArticleEukaryotic cell2005
Pairwise knockdowns of cdc2-related kinases (CRKs) in Trypanosoma brucei identified the CRKs for G1/S and G2/M transitions and demonstrated distinctive cytokinetic regulations between two developmental stages of the organism.
Article in Eukaryotic cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 41 citations in OpenAlex.
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- The RESTRICTION checkpoint: a window of opportunity governing developmental transitions in Toxoplasma gondii.Current opinion in microbiology · 2020Review
- Phylogenetic analysis of cell-cycle regulatory proteins within the Symbiodiniaceae.Scientific reports · 2020Article
- Rapid block of pre-mRNA splicing by chemical inhibition of analog-sensitive CRK9 in Trypanosoma brucei.Molecular microbiology · 2020Article
- Unveiling the Kinomes ofComputational and structural biotechnology journal · 2019Article
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- Proteomic Analysis of the Cell Cycle of Procylic FormMolecular & cellular proteomics : MCP · 2018Article
- AEE788 Inhibits Basal Body Assembly and Blocks DNA Replication in the African Trypanosome.Molecular pharmacology · 2017Article
- Cyclin-Dependent Kinase CRK9, Required for Spliced Leader trans Splicing of Pre-mRNA in Trypanosomes, Functions in a Complex with a New L-Type Cyclin and a Kinetoplastid-Specific Protein.PLoS pathogens · 2016Article
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- Regulation of the cell division cycle in Trypanosoma brucei.Eukaryotic cell · 2012Review
- High-throughput analysis of an RNAi library identifies novel kinase targets in Trypanosoma brucei.Chemical biology & drug design · 2011Article
- 2,4-Diaminopyrimidines as potent inhibitors of Trypanosoma brucei and identification of molecular targets by a chemical proteomics approach.PLoS neglected tropical diseases · 2011Article
- The phosphoproteome of bloodstream form Trypanosoma brucei, causative agent of African sleeping sickness.Molecular & cellular proteomics : MCP · 2009Article
- Identification and specific localization of tyrosine-phosphorylated proteins in Trypanosoma brucei.Eukaryotic cell · 2009Article
- Glycogen synthase kinase 3 is a potential drug target for African trypanosomiasis therapy.Antimicrobial agents and chemotherapy · 2008Article
- Functional characterization of cohesin subunit SCC1 in Trypanosoma brucei and dissection of mutant phenotypes in two life cycle stages.Molecular microbiology · 2008Article
- Effects of Wei Chang An on expression of multiple genes in human gastric cancer grafted onto nude mice.World journal of gastroenterology · 2008Article
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2 authors at 1 institution in 1 country.
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Abstract
Expression of the cdc2-related kinase 3 (CRK3) together with expression of CRK1, -2, -4, or -6, were knocked down in pairs in the procyclic and bloodstream forms of Trypanosoma brucei, using the RNA interference technique. Double knockdowns of CRK3 and CRK2, CRK4, or CRK6 exerted significant growth inhibition and enriched the cells in G2/M phase, whereas a CRK3 plus CRK1 (CRK3 + CRK1) knockdown arrested cells in both G1/S and G2/M transitions. Thus, CRK1 and CRK3 are apparently the kinases regulating the G1/S and G2/M checkpoint passages, respectively, whereas the other CRKs are probably playing only minor roles in cell cycle regulation. A CRK1 + CRK2 knockdown in the procyclic form was found to cause aberrant posterior cytoskeletal morphogenesis (X. M. Tu and C. C. Wang, Mol. Biol. Cell 16:97-105, 2005). A CRK3 + CRK2 knockdown, however, did not lead to such a change, suggesting that CRK2 depletion can lead to the abnormal morphogenesis only when procyclic-form cells are arrested in the G1 phase. The G2/M-arrested procyclic form produces up to 20% stumpy anucleated cells (zoids) in the population, suggesting that cytokinesis and cell division are not blocked by mitotic arrest but are apparently driven to completion by the kinetoplast cycle. In the bloodstream form, however, G2/M arrest resulted in little zoid formation but, instead, enriched a population of cells each containing multiple kinetoplasts, basal bodies, and flagella and an aggregate of multiple nuclei, indicating failure in entering cytokinesis. The two different cytokinetic regulations between two distinct stage-specific forms of the same organism may provide an interesting and useful model for further understanding the evolution of cytokinetic control among eukaryotes.
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