ArticleEukaryotic cell2005
Combinatorial repression of the hypoxic genes of Saccharomyces cerevisiae by DNA binding proteins Rox1 and Mot3.
Article in Eukaryotic cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 55 citations in OpenAlex.
- Alterations to the yeast chromatin landscape during hypoxia.bioRxiv : the preprint server for biology · 2026Article
- Role of eIF5A in Mitochondrial Function.International journal of molecular sciences · 2022Review
- Metabolic engineering strategies for de novo biosynthesis of sterols and steroids in yeast.Bioresources and bioprocessing · 2021Review
- The HMGB ProteinBiomolecules · 2021Article
- New insights on key genes involved in drought stress response of barley: gene networks reconstruction, hub, and promoter analysis.Journal, genetic engineering & biotechnology · 2021Article
- Yeast Translation Elongation Factor eIF5A Expression Is Regulated by Nutrient Availability through Different Signalling Pathways.International journal of molecular sciences · 2020Article
- Assessing regulatory features of the current transcriptional network of Saccharomyces cerevisiae.Scientific reports · 2020Article
- Regulation of Ergosterol Biosynthesis inGenes · 2020Review
- Increased heme synthesis in yeast induces a metabolic switch from fermentation to respiration even under conditions of glucose repression.The Journal of biological chemistry · 2017Article
- Binding to RNA regulates Set1 function.Cell discovery · 2017Article
- Decoupling of divergent gene regulation by sequence-specific DNA binding factors.Nucleic acids research · 2015Article
- Leukemia and ABC transporters.Advances in cancer research · 2015Review
- Changes in transcription and metabolism during the early stage of replicative cellular senescence in budding yeast.The Journal of biological chemistry · 2014Article
- Activator and repressor functions of the Mot3 transcription factor in the osmostress response of Saccharomyces cerevisiae.Eukaryotic cell · 2013Article
- Kluyveromyces lactis: a suitable yeast model to study cellular defense mechanisms against hypoxia-induced oxidative stress.Oxidative medicine and cellular longevity · 2012Review
- The general transcriptional repressor Tup1 is required for dimorphism and virulence in a fungal plant pathogen.PLoS pathogens · 2011Article
- Heme biosynthesis and its regulation: towards understanding and improvement of heme biosynthesis in filamentous fungi.Applied microbiology and biotechnology · 2011Review
- The Rag4 glucose sensor is involved in the hypoxic induction of KlPDC1 gene expression in the yeast Kluyveromyces lactis.Eukaryotic cell · 2011Article
- Metabolic changes through hypoxia in humans and in yeast as a comparable cell model.Sleep & breathing = Schlaf & Atmung · 2010Review
- Regulation of hypoxia adaptation: an overlooked virulence attribute of pathogenic fungi?Medical mycology · 2010Review
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The hypoxic genes of Saccharomyces cerevisiae are transcriptionally repressed during aerobic growth through recruitment of the Ssn6/Tup1 general repression complex by the DNA binding protein Rox1. A second DNA binding protein Mot3 enhances repression of some hypoxic genes. Previous studies characterized the role of Mot3 at the hypoxic ANB1 gene as promoting synergy among one Mot3 site and two Rox1 sites comprising operator A of that gene. Here we studied the role of Mot3 in enhancing repression by Rox1 at another hypoxic gene, HEM13, which is less strongly regulated than ANB1 and has a very different arrangement of Rox1 and Mot3 binding sites. By assessing the effects of deleting Rox1 and Mot3 sites individually and in combination, we found that the major repression of HEM13 occurred through three Mot3 sites closely spaced with a single Rox1 site. While the Mot3 sites functioned additively, they enhanced repression by the single Rox1 site, and the presence of Rox1 enhanced the additive effects of the Mot3 sites. In addition, using a Rox1-Ssn6 fusion protein, we demonstrated that Mot3 enhances Rox1 repression through helping recruit the Ssn6/Tup1 complex. Chromatin immunoprecipitation assays indicated that Rox1 stabilized Mot3 binding to DNA. Integrating these results, we were able to devise a set of rules that govern the combinatorial interactions between Rox1 and Mot3 to achieve differential repression.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.