ArticleClinical and diagnostic laboratory immunology2005
Differential detection of five mouse-infecting helicobacter species by multiplex PCR.
Article in Clinical and diagnostic laboratory immunology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed.
- Prevalence and diversity of Helicobacter species in captive wild carnivores, and their implications for conservation management of endangered species.BMC veterinary research · 2025Article
- Microbiological assessment of Suncus murinus bred and managed as laboratory animals.The Journal of veterinary medical science · 2024Article
- PCR and RT-PCR in the Diagnosis of Laboratory Animal Infections and in Health Monitoring.Journal of the American Association for Laboratory Animal Science : JAALAS · 2020Article
- Immune and microRNA responses toWorld journal of gastroenterology · 2020Article
- Core 1-derived mucin-type O-glycosylation protects against spontaneous gastritis and gastric cancer.The Journal of experimental medicine · 2020Article
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- Lamellipodin-Deficient Mice: A Model of Rectal Carcinoma.PloS one · 2016Article
- Isolation of Helicobacter spp. from mice with rectal prolapses.Comparative medicine · 2014Article
- A mixed population of Helicobacter pylori, Helicobacter bizzozeronii and "Helicobacter heilmannii" in the gastric mucosa of a domestic cat.Irish veterinary journal · 2014Article
- Rapid identification and subtyping of Helicobacter cinaedi strains by intact-cell mass spectrometry profiling with the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry.Journal of clinical microbiology · 2014Article
- Helicobacter hepaticus increases intestinal injury in a rat model of necrotizing enterocolitis.American journal of physiology. Gastrointestinal and liver physiology · 2013Article
- High prevalence and species diversity of Helicobacter spp. detected in wild house mice.Applied and environmental microbiology · 2012Article
- The transcription factor T-bet regulates intestinal inflammation mediated by interleukin-7 receptor+ innate lymphoid cells.Immunity · 2012Article
- Suppression of Tumorigenicity-14, encoding matriptase, is a critical suppressor of colitis and colitis-associated colon carcinogenesis.Oncogene · 2012Article
- Microbial and histopathologic considerations in the use of mouse models of inflammatory bowel diseases.Inflammatory bowel diseases · 2012Review
- Prevalence of murine Helicobacter spp. Infection is reduced by restocking research colonies with Helicobacter-free mice.Journal of the American Association for Laboratory Animal Science : JAALAS · 2012Article
- Expression of the blood-group-related glycosyltransferase B4galnt2 influences the intestinal microbiota in mice.The ISME journal · 2012Article
- Evaluation of a commercial colorimetric fecal dipstick assay for the detection of Helicobacter hepaticus infections in laboratory mice.Journal of the American Association for Laboratory Animal Science : JAALAS · 2010Article
- Effects of Helicobacter infection on research: the case for eradication of Helicobacter from rodent research colonies.Comparative medicine · 2009Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Several species of helicobacter have been isolated from laboratory mice, including H. bilis, H. hepaticus, H. muridarum, H. rodentium, and H. typhlonius, which appear to be the most common. The most widely used published method for molecular detection of these agents is PCR amplification of a conserved region of 16S rRNA, but differential speciation requires restriction enzyme digestion of the amplicons. This study was undertaken to determine PCR conditions that would simultaneously and specifically identify each of the five common species without restriction enzyme analyses. First, we designed novel and specific PCR primers for H. bilis, H. hepaticus, H. muridarum, H. rodentium, and H. typhlonius, using sequences from the heterologous regions of 16S rRNA. Because of comigration of amplified products, we next identified P17, an H. bilis-specific protein; P25, an H. hepaticus-specific protein; and P30, an H. muridarum-specific protein by screening genomic DNA expression libraries of each species. Primers were designed from these three genes, plus newly designed, species-specific 16S rRNA primers for H. rodentium and H. typhlonius that could be utilized for a five-plex PCR. The sizes of the amplicons from H. bilis, H. hepaticus, H. muridarum, H. rodentium, and H. typhlonius were 435, 705, 807, 191, and 122 bp, respectively, allowing simultaneous detection and effective discrimination among species.
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