ArticleClinical and diagnostic laboratory immunology2005
Simultaneous serodetection of 10 highly prevalent mouse infectious pathogens in a single reaction by multiplex analysis.
Article in Clinical and diagnostic laboratory immunology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 51 citations in OpenAlex.
- Long-term storage stability of multiplex immunochromatographic assay strips for serologic monitoring of infectious diseases in laboratory rodents.The Journal of veterinary medical science · 2026Article
- Comparison of Antigen Conjugation to a Peptidic Carrier or to Bovine Serum Albumin in the Serodiagnosis of Canine Visceral Leishmaniasis via Suspension Array Technology.Antibodies (Basel, Switzerland) · 2025Article
- Simultaneous serodetection of major rat infectious pathogens by a multiplex immunochromatographic assay.Experimental animals · 2021Article
- Evaluation of multi-antigen serological screening for active tuberculosis among people living with HIV.PloS one · 2020Article
- Multiplex Immunochromatographic Assay for Serologic Diagnosis of Major Infectious Diseases in Laboratory Mice.Journal of the American Association for Laboratory Animal Science : JAALAS · 2019Article
- Development of a duplex real-time RT-PCR for the simultaneous detection and differentiation of Theiler's murine encephalomyelitis virus and rat theilovirus.Journal of virological methods · 2016Article
- Data mining strategies to improve multiplex microbead immunoassay tolerance in a mouse model of infectious diseases.PloS one · 2015Article
- Assessment of autoantibodies to meningioma in a population-based study.American journal of epidemiology · 2013Article
- Multiplex fluorescent immunoassay for detection of mice infected with lactate dehydrogenase elevating virus.Journal of the American Association for Laboratory Animal Science : JAALAS · 2013Article
- Plasma antibody profiles as diagnostic biomarkers for tuberculosis.Clinical and vaccine immunology : CVI · 2011Article
- Simultaneous detection of antibodies to mouse hepatitis virus recombinant structural proteins by a microsphere-based multiplex fluorescence immunoassay.Clinical and vaccine immunology : CVI · 2011Article
- Serologic prevalence of MPV1 in mouse strains in a commercial laboratory mouse colony determined by using VP1 antigen.Journal of the American Association for Laboratory Animal Science : JAALAS · 2010Article
- Strain- and age-associated variation in viral persistence and antibody response to mouse parvovirus 1 in experimentally infected mice.Journal of the American Association for Laboratory Animal Science : JAALAS · 2010Article
- Profiling antibodies to Mycobacterium tuberculosis by multiplex microbead suspension arrays for serodiagnosis of tuberculosis.Clinical and vaccine immunology : CVI · 2008Article
- Use of a standardized bovine serum panel to evaluate a multiplexed nonstructural protein antibody assay for serological surveillance of foot-and-mouth disease.Clinical and vaccine immunology : CVI · 2007Article
- Multiplex assay for simultaneous measurement of antibodies to multiple Plasmodium falciparum antigens.Clinical and vaccine immunology : CVI · 2006Article
- Simultaneous detection of antibodies to six nonhuman-primate viruses by multiplex microbead immunoassay.Clinical and vaccine immunology : CVI · 2006Article
- Development of a microsphere-based serologic multiplexed fluorescent immunoassay and a reverse transcriptase PCR assay to detect murine norovirus 1 infection in mice.Clinical and diagnostic laboratory immunology · 2005Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Under current practices of mouse colony maintenance, sera from mice are analyzed for antibodies against several widespread infectious pathogens by conventional immunoassays, generally enzyme-linked immunosorbent assay (ELISA). To test for multiple agents, these methods consume large volumes of mouse serum and are laborious and time-consuming. More efficient immunoassays, using small amounts of sample, are therefore needed. Accordingly, we have developed a novel multiplex diagnostic system that employs fluorescent microbeads, coated with purified antigens, for simultaneous serodetection of 10 mouse infectious agents. Individually identifiable, fluorescent microbeads were coated with antigens from Sendai virus, mouse hepatitis virus, Theiler's mouse encephalomyelitis virus/GDVII strain, mouse minute virus, mouse cytomegalovirus, respiratory enteric orphan virus (Reo-3 virus), mouse parvovirus, calf rotavirus for epizootic diarrhea virus of infant mice, vaccinia virus for ectromelia virus, and Mycoplasma pulmonis. Standard sera, singly positive for antibodies to individual infectious agents, were generated by inoculation of BALB/cj and C57BL/6j mice. Sera from these experimentally infected mice, as well as sera from naturally infected mice, were analyzed using a mixture of microbeads coated with antigens of the 10 infectious agents listed above. Results demonstrated that the multiplex assay was at least as sensitive and specific as ELISA for serodetection. Importantly, the multiplex assay required only 1 microliter of serum for simultaneous serodetection of the 10 mouse infectious agents in one reaction vessel. Thus, this multiplex microbead assay is a reliable, efficient, and cost-effective diagnostic modality that will impact serosurveillance of mice used in research.
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