ArticleNucleic acids research2005
Site-specific reverse splicing of a HEG-containing group I intron in ribosomal RNA.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 32 citations in OpenAlex.
- Group I introns: Structure, splicing and their applications in medical mycology.Genetics and molecular biology · 2024Article
- Organellar Introns in Fungi, Algae, and Plants.Cells · 2021Review
- A Phylogenetic Approach to Structural Variation in Organization of Nuclear Group I Introns and Their Ribozymes.Non-coding RNA · 2021Article
- Analysis of small and large subunit rDNA introns from several ectomycorrhizal fungi species.PloS one · 2021Article
- Polymorphism in Mitochondrial Group I Introns amongFrontiers in microbiology · 2018Article
- Accumulation of Stable Full-Length Circular Group I Intron RNAs during Heat-Shock.Molecules (Basel, Switzerland) · 2016Article
- Review
- Bacterial group I introns: mobile RNA catalysts.Mobile DNA · 2014Article
- Molecular characterization of a new member of the lariat capping twin-ribozyme introns.Mobile DNA · 2014Article
- Nuclear group I introns in self-splicing and beyond.Mobile DNA · 2013Article
- Post-transcriptional control of chloroplast gene expression.Gene regulation and systems biology · 2009Article
- Survey of group I and group II introns in 29 sequenced genomes of the Bacillus cereus group: insights into their spread and evolution.Nucleic acids research · 2008Article
- Short-term sequence evolution and vertical inheritance of the Naegleria twin-ribozyme group I intron.BMC evolutionary biology · 2006Article
- Phylogenetic analyses suggest reverse splicing spread of group I introns in fungal ribosomal DNA.BMC evolutionary biology · 2005Article
- The recent transfer of a homing endonuclease gene.Nucleic acids research · 2005Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The wide, but scattered distribution of group I introns in nature is a result of two processes; the vertical inheritance of introns with or without losses, and the occasional transfer of introns across species barriers. Reversal of the group I intron self-splicing reaction, termed reverse splicing, coupled with reverse transcription and genomic integration potentially mediate an RNA-based intron mobility pathway. Compared to the well characterized endonuclease-mediated intron homing, reverse splicing is less specific and represents a likely explanation for many intron transpositions into new genomic sites. However, the frequency and general role of an RNA-based mobility pathway in the spread of natural group I introns is still unclear. We have used the twin-ribozyme intron (Dir.S956-1) from the myxomycete Didymium iridis to test how a mobile group I intron containing a homing endonuclease gene (HEG) selects between potential insertion sites in the small subunit (SSU) rRNA in vitro, in Escherichia coli and in yeast. Surprisingly, the results show a site-specific RNA-based targeting of Dir.S956-1 into its natural (S956) SSU rRNA site. Our results suggest that reverse splicing, in addition to the established endonuclease-mediated homing mechanism, potentially accounts for group I intron spread into the homologous sites of different strains and species.
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