ArticleNucleic acids research2005
Proximity extension of circular DNA aptamers with real-time protein detection.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed.
- In situ imaging of proximal biomolecules via Proximity Anchored Modules Assembly.Journal of nanobiotechnology · 2026Article
- Recent Advances in the Development of Functional Nucleic Acid Biosensors Based on Aptamer-Rolling Circle Amplification.Molecules (Basel, Switzerland) · 2025Review
- Nanomaterial-based biosensors: a new frontier in plant pathogen detection and plant disease management.Frontiers in bioengineering and biotechnology · 2025Review
- Adaptation of a Model Spike Aptamer for Isothermal Amplification-Based Sensing.Sensors (Basel, Switzerland) · 2024Article
- Extending the dynamic range of biomarker quantification through molecular equalization.Nature communications · 2023Article
- Assay methods based on proximity-enhanced reactions for detecting non-nucleic acid molecules.Frontiers in bioengineering and biotechnology · 2023Review
- Single-stranded circular DNA theranostics.Theranostics · 2022Review
- Evolution of a highly functional circular DNA aptamer in serum.Nucleic acids research · 2020Article
- Integrative single-cell analysis.Nature reviews. Genetics · 2019Review
- No Structure-Switching Required: A Generalizable Exonuclease-Mediated Aptamer-Based Assay for Small-Molecule Detection.Journal of the American Chemical Society · 2018Article
- Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Single-cell protein-mRNA correlation analysis enabled by multiplexed dual-analyte co-detection.Scientific reports · 2017Article
- Quantifying Aptamer-Protein Binding via Thermofluorimetric Analysis.Analytical methods : advancing methods and applications · 2015Article
- BIOPHYSICAL PROPERTIES OF NUCLEIC ACIDS AT SURFACES RELEVANT TO MICROARRAY PERFORMANCE.Biomaterials science · 2014Article
- Nucleic acids for ultra-sensitive protein detection.Sensors (Basel, Switzerland) · 2013Review
- Fluorescent DNA-based enzyme sensors.Chemical Society reviews · 2011Review
- Fluorescence protection assay: a novel homogeneous assay platform toward development of aptamer sensors for protein detection.Nucleic acids research · 2011Article
- Homogeneous antibody-based proximity extension assays provide sensitive and specific detection of low-abundant proteins in human blood.Nucleic acids research · 2011Article
- Aptamer-based fluorescent biosensors.Current medicinal chemistry · 2011Review
- Functional nucleic acid sensors.Chemical reviews · 2009Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Multivalent circular aptamers or 'captamers' have recently been introduced through the merger of aptameric recognition functions with the basic principles of DNA nanotechnology. Aptamers have strong utility as protein-binding motifs for diagnostic applications, where their ease of discovery, thermal stability and low cost make them ideal components for incorporation into targeted protein assays. Here we report upon a property specific to circular DNA aptamers: their intrinsic compatibility with a highly sensitive protein detection method termed the 'proximity extension' assay. The circular DNA architecture facilitates the integration of multiple functional elements into a single molecule: aptameric target recognition, nucleic acid hybridization specificity and rolling circle amplification. Successful exploitation of these properties is demonstrated for the molecular analysis of thrombin, with the assay delivering a detection limit nearly three orders of magnitude below the dissociation constants of the two contributing aptamer-thrombin interactions. Real-time signal amplification and detection under isothermal conditions points towards potential clinical applications, with both fluorescent and bioelectronic methods of detection achieved. This application elaborates the pleiotropic properties of circular DNA aptamers beyond the stability, potency and multitargeting characteristics described earlier.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.