ArticleMolecular genetics and genomics : MGG2005
An Arabidopsis mutant that is resistant to the protoporphyrinogen oxidase inhibitor acifluorfen shows regulatory changes in tetrapyrrole biosynthesis.
Article in Molecular genetics and genomics : MGG, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 34 citations in OpenAlex.
- The function of tea plant CsMORF9.3 in chlorophyll biosynthesis and RNA editing.Plant cell reports · 2026Article
- The Synergistic Mechanism of Photosynthesis and Antioxidant Metabolism between the Green and White Tissues ofInternational journal of molecular sciences · 2023Article
- Identification and haplotype analysis of SiCHLI: a gene for yellow-green seedling as morphological marker to accelerate foxtail millet (Setaria italica) hybrid breeding.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Identification and function analysis of yellow-leaf mutant (YX-yl) of broomcorn millet.BMC plant biology · 2022Article
- A point mutation in the photosystem I P700 chlorophyll a apoprotein A1 gene confers variegation in Helianthus annuus L.Plant molecular biology · 2020Article
- Fine mapping and candidate gene analysis of the virescent gene vMolecular genetics and genomics : MGG · 2018Article
- The chlorophyll-deficient golden leaf mutation in cucumber is due to a single nucleotide substitution in CsChlI for magnesium chelatase I subunit.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2016Article
- Efficient transformation and artificial miRNA gene silencing in Lemna minor.Plant biology (Stuttgart, Germany) · 2015Article
- Identical substitutions in magnesium chelatase paralogs result in chlorophyll-deficient soybean mutants.G3 (Bethesda, Md.) · 2014Article
- Increased expression of Fe-chelatase leads to increased metabolic flux into heme and confers protection against photodynamically induced oxidative stress.Plant molecular biology · 2014Article
- Article
- Tetrapyrrole Metabolism in Arabidopsis thaliana.The arabidopsis book · 2011Article
- Arabidopsis CHLI2 can substitute for CHLI1.Plant physiology · 2009Article
- Suppression of the barley uroporphyrinogen III synthase gene by a Ds activation tagging element generates developmental photosensitivity.The Plant cell · 2009Article
- Recent overview of the Mg branch of the tetrapyrrole biosynthesis leading to chlorophylls.Photosynthesis research · 2008Review
- The analysis of the ChlI 1 and ChlI 2 genes using acifluorfen-resistant mutant of Arabidopsis thaliana.Planta · 2007Article
- Rice Chlorina-1 and Chlorina-9 encode ChlD and ChlI subunits of Mg-chelatase, a key enzyme for chlorophyll synthesis and chloroplast development.Plant molecular biology · 2006Article
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8 authors at 2 institutions in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
Several Arabidopsis mutants of the ecotype Dijon were isolated that show resistance to the herbicide acifluorfen, which inactivates protoporphyrinogen oxidase (PPOX), an enzyme of tetrapyrrole biosynthesis. This enzyme provides protoporphyrin for both Mg chelatase and ferrochelatase at the branchpoint, which leads to chlorophyll and heme, respectively. One of the mutations, aci5-3, displays semidominant inheritance. Heterozygous progeny showed yellow-green leaves, while the homozygous seedlings were white and inviable, but could be rescued by supplementing the medium with sugar. Interestingly, the expression of neither of the two forms of PPOX was altered in the mutant, but the rate of synthesis of 5-aminolevulinate, the precursor of all tetrapyrroles, was drastically reduced. Genetic mapping revealed the mutant locus is closely linked to the ch42 marker, which is itself located in the CHLI-1 gene which codes for one of the three subunits of Mg chelatase. The cs mutant also shows a defect in this gene, and test for allelism with aci5-3 confirmed that the two mutations are allelic. Sequencing of the wild type and aci5-3 alleles of CHLI-1 revealed a single base change (G718A), which results in a D240N substitution in the CHLI-1 protein. In the homozygous aci5-3 mutant no CHLI-1 RNA or protein could be detected. Strikingly, CHLH and CHLI-2 transcripts were also absent. This indicates the existence of a feedback-regulatory mechanism that inactivates the genes encoding certain Mg chelatase subunits. The basis for the semidominant inheritance pattern and the relationship between herbicide resistance and modified gene expression is discussed.
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