ArticleJournal of clinical microbiology2005
Detection and genotyping of Mycobacterium species from clinical isolates and specimens by oligonucleotide array.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Biochip-Based Identification of Mycobacterial Species in Russia.International journal of molecular sciences · 2024Article
- The Intriguing Pattern of Nontuberculous Mycobacteria in Bulgaria and Description ofInternational journal of molecular sciences · 2024Article
- Evaluation of a low-density hydrogel microarray technique for mycobacterial species identification.Journal of clinical microbiology · 2015Article
- Demonstrating a multi-drug resistant Mycobacterium tuberculosis amplification microarray.Journal of visualized experiments : JoVE · 2014Article
- Evaluation of peptide nucleic acid probe-based real-time PCR for detection of Mycobacterium tuberculosis complex and nontuberculous mycobacteria in respiratory specimens.Annals of laboratory medicine · 2012Article
- Biochip system for rapid and accurate identification of mycobacterial species from isolates and sputum.Journal of clinical microbiology · 2010Article
- Development and evaluation of oligonucleotide chip based on the 16S-23S rRNA gene spacer region for detection of pathogenic microorganisms associated with sepsis.Journal of clinical microbiology · 2010Article
- Identification of legionella species by use of an oligonucleotide array.Journal of clinical microbiology · 2009Article
- Use of a novel multiplex probe array for rapid identification of Mycobacterium species from clinical isolates.World journal of microbiology & biotechnology · 2007Article
- Mycobacterium simiae complex infection in an immunocompetent child.Journal of clinical microbiology · 2007Review
- Fluorescent nanoparticle-based indirect immunofluorescence microscopy for detection of Mycobacterium tuberculosis.Journal of biomedicine & biotechnology · 2007Article
- Array-based identification of species of the genera Abiotrophia, Enterococcus, Granulicatella, and Streptococcus.Journal of clinical microbiology · 2006Article
- Use of PCR and reverse line blot hybridization macroarray based on 16S-23S rRNA gene internal transcribed spacer sequences for rapid identification of 34 mycobacterium species.Journal of clinical microbiology · 2006Article
- Evaluation of the GenoType Mycobacterium Assay for identification of mycobacterial species from cultures.Journal of clinical microbiology · 2006Article
- Comparison of a conventional antimicrobial susceptibility assay to an oligonucleotide chip system for detection of drug resistance in Mycobacterium tuberculosis isolates.Journal of clinical microbiology · 2006Article
- Querying the public databases for sequences using complex keywords contained in the feature lines.BMC bioinformatics · 2006Article
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9 authors.
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Abstract
Identification of pathogenic Mycobacterium species is important for a successful diagnosis of mycobacteriosis. The purpose of this study was to develop an oligonucleotide array which could detect and differentiate mycobacteria to the species level by using the internal transcribed spacer (ITS) sequence. Using a genus-specific probe and 20 species-specific probes including two M. avium-intracellulare complex (MAC)-specific probes, we have developed an ITS-based oligonucleotide array for the rapid and reliable detection and discrimination of M. tuberculosis, MAC, M. fortuitum, M. chelonae, M. abscessus, M. kansasii, M. gordonae, M. scrofulaceum, M. szulgai, M. vaccae, M. xenopi, M. terrae, M. flavescens, M. smegmatis, M. malmoense, M. simiae, M. marinum, M. ulcerans, M. gastri, and M. leprae. All mycobacteria were hybridized with a genus-specific probe (PAN-03) for detection of the genus Mycobacterium. Mycobacterial species were expected to show a unique hybridization pattern with species-specific probes, except for M. marinum and M. ulcerans, which were not differentiated by ITS-based probe. Among the species-specific probes, two kinds of species-specific probes were designed for MAC in which there were many subspecies. The performance of the oligonucleotide array assay was demonstrated by using 46 reference strains, 149 clinical isolates, and 155 clinical specimens. The complete procedure (DNA extraction, PCR, DNA hybridization, and scanning) was carried out in 4.5 h. Our results indicated that the oligonucleotide array is useful for the identification and discrimination of mycobacteria from clinical isolates and specimens in an ordinary clinical laboratory.
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