ArticleJournal of clinical microbiology2005
Loop-mediated isothermal amplification method targeting the lytA gene for detection of Streptococcus pneumoniae.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Comparative Genotyping of Malaysian Clinical Isolates ofThe Malaysian journal of medical sciences : MJMS · 2025Article
- Rapid detection ofFrontiers in cellular and infection microbiology · 2025Article
- A novel LAMP-based assay for the identification ofJournal of clinical microbiology · 2024Article
- Development and evaluation of a real-time multienzyme isothermal rapid amplification assay for rapid detection of Streptococcus pneumoniae.Scientific reports · 2024Article
- Comparison of pneumococcal colonization density among healthy children and children with respiratory symptoms using real time PCR (RT-PCR).BMC microbiology · 2022Article
- Antimicrobial susceptibility, serotype distribution, virulence profile and molecular typing of piliated clinical isolates of pneumococci from east coast, Peninsular Malaysia.Scientific reports · 2021Article
- Validation of a Loop-Mediated Isothermal Amplification Assay for Rapid Diagnosis of Invasive Pneumococcal Disease.Frontiers in cellular and infection microbiology · 2020Article
- Establishment of loop-mediated isothermal amplification for rapid detection ofExperimental and therapeutic medicine · 2019Article
- Molecular Characterization of Pneumococcal Surface Protein A (PspA), Serotype Distribution and Antibiotic Susceptibility of Streptococcus pneumoniae Strains Isolated from Pakistan.Infectious diseases and therapy · 2018Article
- Loop-mediated isothermal amplification (LAMP): a versatile technique for detection of micro-organisms.Journal of applied microbiology · 2018Review
- Loop-Mediated Isothermal Amplification Methods for Diagnosis of Bacterial Meningitis.Frontiers in pediatrics · 2018Review
- Rapid visual detection of binary toxin producingExperimental and therapeutic medicine · 2017Article
- Visual Detection of Enterohemorrhagic Escherichia coli O157:H7 Using Loop-Mediated Isothermal Amplification.Electronic physician · 2016Article
- Fatal infection in adults by pneumolysin & autolysin producing, non-vaccine serotype Streptococcus pneumonia.The Indian journal of medical research · 2016Article
- Emergence of Neoteric Serotypes Among Multidrug Resistant Strains of Streptococcus pneumoniae Prevalent in Egypt.Jundishapur journal of microbiology · 2016Article
- Development of a rapid recombinase polymerase amplification assay for the detection of Streptococcus pneumoniae in whole blood.BMC infectious diseases · 2015Article
- Development of Primer Sets for Loop-Mediated Isothermal Amplification that Enables Rapid and Specific Detection of Streptococcus dysgalactiae, Streptococcus uberis and Streptococcus agalactiae.International journal of environmental research and public health · 2015Article
- A Comparison of In-House Real-Time LAMP Assays with a Commercial Assay for the Detection of Pathogenic Bacteria.Molecules (Basel, Switzerland) · 2015Article
- Standardisation and evaluation of a quantitative multiplex real-time PCR assay for the rapid identification ofPneumonia (Nathan Qld.) · 2015Article
- A versatile PDMS/paper hybrid microfluidic platform for sensitive infectious disease diagnosis.Analytical chemistry · 2014Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is difficult to separate Streptococcus pneumoniae from the genotypically similar species Streptococcus mitis and Streptococcus oralis, which are commensals of the human oral cavity. A novel nucleic acid amplification technique, loop-mediated isothermal amplification (LAMP), which amplifies DNA under isothermal conditions (63 degrees C) with high specificity, efficiency, and rapidity, was examined regarding its applicability for detecting S. pneumoniae. An S. pneumoniae-specific LAMP primer targeting the lytA gene was designed. The primer specificity was validated using 10 Streptococcus and 7 non-Streptococcus species. Within 60 min, the assay could detect 10 or more copies of purified S. pneumoniae DNA with a sensitivity 1,000 times that of conventional PCR. Clinical isolates of 21 other strains (3 S. oralis, 17 S. mitis, and 1 Streptococcus species) that harbor virulence-factor-encoding genes (lytA or ply) were tried to differentiate S. pneumoniae. The detection of S. pneumoniae in clinical isolates was more selective using the LAMP method than using conventional PCR. Therefore, LAMP appears to be a sensitive and reliable means of diagnosing S. pneumoniae infection.
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