ArticleThe Biochemical journal2005
Regulation of ecdysteroid signalling during Drosophila development: identification, characterization and modelling of ecdysone oxidase, an enzyme involved in control of ligand concentration.
Article in The Biochemical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 60 citations in OpenAlex.
- Article
- Decoding the general role of tRNA queuosine modification in eukaryotes.Scientific reports · 2025Article
- A midgut transcriptional regulatory loop favors an insect host to withstand a bacterial pathogen.Innovation (Cambridge (Mass.)) · 2024Article
- Cis-regulatory polymorphism at fiz ecdysone oxidase contributes to polygenic evolutionary response to malnutrition in Drosophila.PLoS genetics · 2024Article
- A dynamical model of growth and maturation inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Exorista sorbillans (Diptera: Tachinidae) parasitism shortens host larvae growth duration by regulating ecdysone and juvenile hormone titers in Bombyx mori (Lepidoptera: Bombycidae).Journal of insect science (Online) · 2023Article
- Poly(ADP-ribosyl)ating enzymes cooperate to coordinate development.Scientific reports · 2022Article
- Chronic exposure to the star polycation (SPc) nanocarrier in the larval stage adversely impairs life history traits in Drosophila melanogaster.Journal of nanobiotechnology · 2022Article
- Genome-wide transcriptomic changes reveal the genetic pathways involved in insect migration.Molecular ecology · 2022Article
- The Genomic Architecture of Adaptation to Larval Malnutrition Points to a Trade-off with Adult Starvation Resistance in Drosophila.Molecular biology and evolution · 2021Article
- The Effect of Diet on Midgut and Resulting Changes in Infectiousness of AcMNPV Baculovirus in the Cabbage Looper,Frontiers in physiology · 2018Article
- Ecdysone oxidase and 3-dehydroecdysone-3β-reductase contribute to the synthesis of ecdysone during early embryonic development of the silkworm.International journal of biological sciences · 2018Article
- The caterpillar fungus, Ophiocordyceps sinensis, genome provides insights into highland adaptation of fungal pathogenicity.Scientific reports · 2017Article
- Genomics and Comparative Genomic Analyses Provide Insight into the Taxonomy and Pathogenic Potential of NovelFrontiers in cellular and infection microbiology · 2017Article
- Recurrent specialization on a toxic fruit in an island Drosophila population.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- The Female Post-Mating Response Requires Genes Expressed in the Secondary Cells of the Male Accessory Gland in Drosophila melanogaster.Genetics · 2016Article
- Ectopic expression of ecdysone oxidase impairs tissue degeneration in Bombyx mori.Proceedings. Biological sciences · 2015Article
- Independently recruited oxidases from the glucose-methanol-choline oxidoreductase family enabled chemical defences in leaf beetle larvae (subtribe Chrysomelina) to evolve.Proceedings. Biological sciences · 2014Article
- Article
- Molecular cloning and characterization of Ecdysone oxidase and 3-dehydroecdysone-3α-reductase involved in the ecdysone inactivation pathway of silkworm, Bombyx mori.International journal of biological sciences · 2012Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The steroidal moulting hormones (ecdysteroids) mediate developmental transitions in insects, and their regulation is mainly controlled by the production and inactivation of these steroid hormones at the appropriate developmental times. One route of metabolism of ecdysteroids in insects involves EO (ecdysone oxidase)-catalysed conversion into 3-dehydroecdysteroid, which undergoes reduction to the corresponding 3-epiecdysteroid. By a twin-stranded bioinformatics approach, employing both phylogenomics and model structure-based analysis, we first predicted that DmEO (the EO of Drosophila melanogaster) corresponds to the protein product of gene CG9504. When CG9504 was expressed in COS7 cells, significant conversion of ecdysone into 3-dehydroecdysone was observed. Quantitative PCR and enzyme assay showed that DmEO was mainly expressed in the midgut during the late instars at a time corresponding to a hormone titre peak. DmEO shares only 27% amino acid sequence identity with Spodoptera littoralis (Lepidoptera) EO, yet key substrate-binding residues are well conserved. A model of DmEO is consistent with an inability to catalyse reaction of cholesterol derivatives. The significance of DmEO in ligand activation is discussed in relation to new evidence suggesting that 3-dehydro- and 3-epiecdysteroids may be functionally active as ligands in a novel, atypical ecdysteroid signalling pathway involving the Drosophila orphan nuclear receptor, DHR38, rather than being merely hormone inactivation products.
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