ArticleMolecular genetics and genomics : MGG2005
Characterization by suppression subtractive hybridization of transcripts that are differentially expressed in leaves of apple scab-resistant and susceptible cultivars of Malus domestica.
Article in Molecular genetics and genomics : MGG, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 83 citations in OpenAlex.
- Phenotyping, genetics, and "-omics" approaches to unravel and introgress enhanced resistance against apple scab (Horticulture research · 2024Article
- Comparative transcriptomics unravels new genes imparting scab resistance in apple (Malus x domestica Borkh.).Functional & integrative genomics · 2022Article
- Resistant and susceptible cacao genotypes exhibit defense gene polymorphism and unique early responses to Phytophthora megakarya inoculation.Plant molecular biology · 2019Article
- A Role for Zinc in Plant Defense Against Pathogens and Herbivores.Frontiers in plant science · 2019Review
- Transcriptomic Response of Resistant (PI613981-Frontiers in plant science · 2017Article
- cDNA-AFLP analysis reveals differential gene expression in incompatible interaction between infected non-heading Chinese cabbage and Hyaloperonospora parasitica.Horticulture research · 2016Article
- Genome-wide identification and expression profiling of the late embryogenesis abundant genes in potato with emphasis on dehydrins.Molecular biology reports · 2015Article
- Genome-wide analysis and expression profiling of the ERF transcription factor family in potato (Solanum tuberosum L.).Molecular biotechnology · 2015Article
- Gene expression profiling by cDNA-AFLP reveals potential candidate genes for partial resistance of 'Président Roulin' against Venturia inaequalis.BMC genomics · 2014Article
- Screening and identification of resistance related proteins from apple leaves inoculated with Marssonina coronaria (EII. & J. J. Davis).Proteome science · 2014Article
- Anatomical and physiological differences and differentially expressed genes between the green and yellow leaf tissue in a variegated chrysanthemum variety.Molecular biotechnology · 2013Article
- RNA-Seq analysis reveals candidate genes for ontogenic resistance in Malus-Venturia pathosystem.PloS one · 2013Article
- Article
- Characterization of resistance to pine wood nematode infection in Pinus thunbergii using suppression subtractive hybridization.BMC plant biology · 2012Article
- Identification and transcriptional profiling of differentially expressed genes associated with resistance to Pseudoperonospora cubensis in cucumber.Plant cell reports · 2011Article
- Article
- Isolation of high quality RNA and construction of a suppression subtractive hybridization library from ramie (Boehmeria nivea L. Gaud.).Molecular biology reports · 2010Article
- Developing microbe-plant interactions for applications in plant-growth promotion and disease control, production of useful compounds, remediation and carbon sequestration.Microbial biotechnology · 2009Review
- Identification and characterization of differentially expressed genes in the resistance reaction in taro infected with Phytophthora colocasiae.Molecular biology reports · 2009Article
- The Venturia apple pathosystem: pathogenicity mechanisms and plant defense responses.Journal of biomedicine & biotechnology · 2009Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In order to compare transcription profiles in cultivars of Malus domestica that are differentially sensitive to apple scab (Venturia inaequalis), two cDNA libraries were constructed using the suppression subtractive hybridization (SSH) method. Subtraction hybridization was performed between cDNAs from uninfected young leaves of the resistant cultivar Remo and the susceptible Elstar. In total, 480 EST clones were obtained: 218 (ELSTAR) clones represent transcripts that are preferentially expressed in Elstar, while the other 262 (REMO) are derived from RNAs that are more highly expressed in Remo. The putative functions of about 50% of the cloned sequences could be identified by sequencing and subsequent homology searches in databases or by dot-blot hybridization to known targets. In the resistant cv. Remo the levels of transcripts encoding a number of proteins related to plant defense (such as beta-1,3-glucanase, ribonuclease-like PR10, cysteine protease inhibitor, endochitinase, ferrochelatase, and ADP-ribosylation factor) or detoxification of reactive oxygen species (such as superoxide dismutase) were highly up-regulated relative to the amounts present in cv. Elstar. Most surprising was the large number of clones derived from mRNAs for metallothioneins of type 3 (91 out of 262) found in the REMO population. The corresponding transcripts were only present in small amounts in young uninfected leaves of the cv. Elstar, but were up-regulated in the susceptible cultivar after inoculation with V. inaequalis. These results indicate that constitutively high-level expression of PR proteins may protect cv. Remo from infection by different plant pathogens.
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