ArticleApplied and environmental microbiology2005
Diversity of transcripts of nitrite reductase genes (nirK and nirS) in rhizospheres of grain legumes.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Co-association of Two nir Denitrifiers Under the Influence of Emergent Macrophytes.Microbial ecology · 2020Article
- Co-occurrence pattern and function prediction of bacterial community in Karst cave.BMC microbiology · 2020Article
- No Tangible Effects of Field-Grown Cisgenic Potatoes on Soil Microbial Communities.Frontiers in bioengineering and biotechnology · 2020Article
- Article
- Field-Scale Pattern of Denitrifying Microorganisms and NMicrobial ecology · 2017Article
- Soil C and N statuses determine the effect of maize inoculation by plant growth-promoting rhizobacteria on nitrifying and denitrifying communities.Scientific reports · 2017Article
- Impacts of Long-Term Irrigation of Domestic Treated Wastewater on Soil Biogeochemistry and Bacterial Community Structure.Applied and environmental microbiology · 2015Article
- Article
- Different land use intensities in grassland ecosystems drive ecology of microbial communities involved in nitrogen turnover in soil.PloS one · 2013Article
- Transitions in nirS-type denitrifier diversity, community composition, and biogeochemical activity along the Chesapeake Bay estuary.Frontiers in microbiology · 2013Article
- Relating Phylogenetic and Functional Diversity among Denitrifiers and Quantifying their Capacity to Predict Community Functioning.Frontiers in microbiology · 2012Article
- Identification of active denitrifiers in rice paddy soil by DNA- and RNA-based analyses.Microbes and environments · 2012Article
- Microbiology of nitrogen cycle in animal manure compost.Microbial biotechnology · 2011Review
- Effect of sulfadiazine-contaminated pig manure on the abundances of genes and transcripts involved in nitrogen transformation in the root-rhizosphere complexes of maize and clover.Applied and environmental microbiology · 2010Article
- Abundance of microbes involved in nitrogen transformation in the rhizosphere of Leucanthemopsis alpina (L.) Heywood grown in soils from different sites of the Damma glacier forefield.Microbial ecology · 2010Article
- Effect of sulfadiazine on abundance and diversity of denitrifying bacteria by determining nirK and nirS genes in two arable soils.Microbial ecology · 2010Article
- Extraction of mRNA from soil.Applied and environmental microbiology · 2010Article
- The impact of using mature compost on nitrous oxide emission and the denitrifier community in the cattle manure composting process.Microbial ecology · 2010Article
- Diversity of nirK denitrifying genes and transcripts in an agricultural soil.Applied and environmental microbiology · 2009Article
- Dynamics of nitrous oxide reductase genes (nosZ) in intertidal rocky biofilms and sediments of the Douro River estuary (Portugal), and their relation to N-biogeochemistry.Microbial ecology · 2008Article
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Authors and funding
5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Transcription of the nirK and nirS genes coding for dissimilatory bacterial nitrite reductases was analyzed by reverse transcription PCR (RT-PCR) of mRNA isolated from rhizosphere samples of three economically important grain legumes at maturity: Vicia faba, Lupinus albus, and Pisum sativum. The nirK gene and transcripts could be detected in all the rhizosphere samples. In contrast, nirS could not be detected. Sampling variations were analyzed by comparing denaturing gradient gel electrophoresis profiles derived from nirK RT-PCR products. High similarity was observed between the replicates, and so one representative product per legume was cloned. Clones with the correct insert size were screened by restriction fragment length polymorphism by using the restriction enzyme MspI. The clones could be distributed into 12 different patterns. Patterns 1, 3, 4, 5, and 7 were common in clone libraries of the three rhizosphere types under study. Patterns 2, 9, 10, and 11 were absent from Pisum rhizospheres, while patterns 6, 8, and 12 were absent from the Vicia library. Pattern 1, which was the most dominant in the Vicia and Lupinus libraries, constituted about 25% of all clones. The Lupinus library had clones representing all 12 patterns, indicating it to be the most diverse among the three. Clones representative of each pattern were sequenced. All patterns grouped together forming a distinct cluster, which was divergent from previously described nirK sequences in the database. The study revealed a hitherto unknown diversity of denitrifiers in legume rhizospheres. A plant-dependent rhizosphere effect on the transcripts of a gene was evident.
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