ArticleApplied and environmental microbiology2005
Genes involved in SkfA killing factor production protect a Bacillus subtilis lipase against proteolysis.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 19 citations in OpenAlex.
- Comparative genomics analysis of Bacillus altitudinis G03 provides insights into its biocontrol and probiotic traits.BMC genomics · 2025Article
- Combining metabolic flux analysis and adaptive evolution to enhance lipase production in Bacillus subtilis.Journal of industrial microbiology & biotechnology · 2019Article
- Article
- Deleting multiple lytic genes enhances biomass yield and production of recombinant proteins by Bacillus subtilis.Microbial cell factories · 2014Article
- Molecular characterization of a proteolysis-resistant lipase from Bacillus pumilus SG2.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2014Article
- Bacillus subtilis: from soil bacterium to super-secreting cell factory.Microbial cell factories · 2013Article
- Effect of temperature on the cannibalistic behavior of Bacillus subtilis.Applied and environmental microbiology · 2008Article
- The H2O2 stress-responsive regulator PerR positively regulates srfA expression in Bacillus subtilis.Journal of bacteriology · 2005Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Small lipases of Bacillus species, such as LipA from Bacillus subtilis, have a high potential for industrial applications. Recent studies showed that deletion of six AT-rich islands from the B. subtilis genome results in reduced amounts of extracellular LipA. Here we demonstrate that the reduced LipA levels are due to the absence of four genes, skfABCD, located in the prophage 1 region. Intact skfABCD genes are required not only for LipA production at wild-type levels by B. subtilis 168 but also under conditions of LipA overproduction. Notably, SkfA has bactericidal activity and, probably, requires the SkfB to SkfD proteins for its production. The present results show that LipA is more prone to proteolytic degradation in the absence of SkfA and that high-level LipA production can be improved significantly by employing multiple protease-deficient B. subtilis strains. In conclusion, our findings imply that SkfA protects LipA, directly or indirectly, against proteolytic degradation. Conceivably, SkfA could act as a modulator in LipA folding or as a protease inhibitor.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.