ArticleApplied and environmental microbiology2005
Rapid and sensitive detection of noroviruses by using TaqMan-based one-step reverse transcription-PCR assays and application to naturally contaminated shellfish samples.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 104 papers.
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Who cites it
104 citing papers in PubMed, 362 citations in OpenAlex.
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- Epidemiological surveillance of astrovirus, norovirus, rotavirus, and enterovirus in sewage (2022-2023) in Giza, Egypt.Journal of water and health · 2025Article
- A new wave of resurgence for GII.4 Sydney in Huzhou, particularly GII.4 Sydney[P16], between 2019 and 2023.BMC infectious diseases · 2025Article
- Assessing the nucleic acid decay of human wastewater markers and enteric viruses in estuarine waters in Sydney, Australia.The Science of the total environment · 2024Article
- Article
- Comparing the decay of human wastewater-associated markers and enteric viruses in laboratory microcosms simulating estuarine waters in a temperate climatic zone using qPCR/RT-qPCR assays.The Science of the total environment · 2024Article
- Molecular and Genetics-Based Systems for Tracing the Evolution and Exploring the Mechanisms of Human Norovirus Infections.International journal of molecular sciences · 2023Review
- Influence of herd immunity on norovirus: a long-term field study of repeated viral gastroenteritis outbreaks at the same facilities.BMC infectious diseases · 2023Article
- Laboratory Diagnosis of HEV Infection.Advances in experimental medicine and biology · 2023Review
- Development and evaluation of a new luciferase immunosorbent assay to detect GII.6 norovirus-specific IgG in different domestic and wild animals.Frontiers in microbiology · 2023Article
- Detection of Human and Fish Viruses in Marine Gastropods.Animals : an open access journal from MDPI · 2022Article
- Effectiveness of two wastewater disinfection strategies for the removal of fecal indicator bacteria, bacteriophage, and enteric viral pathogens concentrated using dead-end hollow fiber ultrafiltration (D-HFUF).The Science of the total environment · 2022Article
- Efficacy of an alcohol-based surface disinfectant formulation against human norovirus.Journal of applied microbiology · 2022Article
- Physicochemical stability and virucidal effect of diluted, slightly acidic electrolyzed water against human norovirus.Food science and biotechnology · 2022Article
- Comparative Assessment of the Efficacy of Commercial Hand Sanitizers Against Human Norovirus Evaluated by anFrontiers in microbiology · 2022Article
- Advances and Future Perspective on Detection Technology of Human Norovirus.Pathogens (Basel, Switzerland) · 2021Review
- COVID-19 Crisis Creates Opportunity towards Global Monitoring & Surveillance.Pathogens (Basel, Switzerland) · 2021Review
- Article
44 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Noroviruses (NoV), which are members of the family Caliciviridae, are the most important cause of outbreaks of acute gastroenteritis worldwide and are commonly found in shellfish grown in polluted waters. In the present study, we developed broadly reactive one-step TaqMan reverse transcription (RT)-PCR assays for the detection of genogroup I (GI) and GII NoV in fecal samples, as well as shellfish samples. The specificity and sensitivity of all steps of the assays were systematically evaluated, and in the final format, the monoplex assays were validated by using RNA extracted from a panel of 84 stool specimens, which included NoV strains representing 19 different genotypes (7 GI, 11 GII, and 1 GIV strains). The assays were further validated with 38 shellfish cDNA extracts previously tested by nested PCR. Comparison with a recently described real-time assay showed that our assay had significantly higher sensitivity and was at least as sensitive as the nested PCR. For stool specimens, a one-step duplex TaqMan RT-PCR assay performed as well as individual genogroup-specific monoplex assays. All other enteric viruses examined were negative, and no cross-reaction between genogroups was observed. These TaqMan RT-PCR assays provide rapid (less than 90 min), sensitive, and reliable detection of NoV and should prove to be useful for routine monitoring of both clinical and shellfish samples.
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