ArticleRNA (New York, N.Y.)2005
UTP-dependent turnover of Trypanosoma brucei mitochondrial mRNA requires UTP polymerization and involves the RET1 TUTase.
Article in RNA (New York, N.Y.), 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 37 citations in OpenAlex.
- Mitochondrial RNA quality control in trypanosomes.Wiley interdisciplinary reviews. RNA · 2021Review
- Probabilistic models of biological enzymatic polymerization.PloS one · 2021Article
- Lexis and Grammar of Mitochondrial RNA Processing in Trypanosomes.Trends in parasitology · 2020Review
- Gene fragmentation and RNA editing without borders: eccentric mitochondrial genomes of diplonemids.Nucleic acids research · 2020Article
- PPR polyadenylation factor defines mitochondrial mRNA identity and stability in trypanosomes.The EMBO journal · 2017Article
- RNA Editing TUTase 1: structural foundation of substrate recognition, complex interactions and drug targeting.Nucleic acids research · 2016Article
- Antisense Transcripts Delimit Exonucleolytic Activity of the Mitochondrial 3' Processome to Generate Guide RNAs.Molecular cell · 2016Article
- Article
- Review
- Dual core processing: MRB1 is an emerging kinetoplast RNA editing complex.Trends in parasitology · 2013Review
- Article
- Functional characterization of two paralogs that are novel RNA binding proteins influencing mitochondrial transcripts of Trypanosoma brucei.RNA (New York, N.Y.) · 2012Article
- Mitochondrial RNA processing in trypanosomes.Research in microbiology · 2011Review
- RET1-catalyzed uridylylation shapes the mitochondrial transcriptome in Trypanosoma brucei.Molecular and cellular biology · 2010Article
- Guide RNA-binding complex from mitochondria of trypanosomatids.Molecular cell · 2008Article
- 3' adenylation determines mRNA abundance and monitors completion of RNA editing in T. brucei mitochondria.The EMBO journal · 2008Article
- Terminal RNA uridylyltransferases of trypanosomes.Biochimica et biophysica acta · 2008Article
- Determinants of substrate specificity in RNA-dependent nucleotidyl transferases.Biochimica et biophysica acta · 2008Review
- Stable PNPase RNAi silencing: its effect on the processing and adenylation of human mitochondrial RNA.RNA (New York, N.Y.) · 2008Article
- Targeted depletion of a mitochondrial nucleotidyltransferase suggests the presence of multiple enzymes that polymerize mRNA 3' tails in Trypanosoma brucei mitochondria.Molecular and biochemical parasitology · 2007Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Trypanosoma brucei mitochondria possess a unique RNA decay pathway in which rapid degradation of polyadenylated mRNAs is dependent on the addition of UTP, as measured by in organello pulse chase assays. To determine the mechanism by which UTP stimulates the degradation of polyadenylated RNAs, we performed in organello pulse chase assays under different conditions. Treatment of mitochondria with proteinase K revealed that UTP does not act through a receptor on the surface of the mitochondria. To determine if the UTP-stimulated RNA decay pathway is triggered by the mitochondrial energy state or ATP:UTP ratio, increasing ATP was added to a constant amount of UTP during the chase period of the assay. Results indicate that rapid turnover is responsive to UTP and not the ATP:UTP ratio. Experiments using UTP analogs demonstrate that UTP polymerization into RNAs is necessary for UTP-dependent degradation. Furthermore, experiments performed with RNAi cells indicate that the RET1 terminal uridylyl transferase (TUTase) is required for UTP-dependent decay of polyadenylated RNAs. Overall, these results show that degradation of polyadenylated RNAs in T. brucei mitochondria can occur through a unique mechanism that requires the polymerization of UTP into RNAs, presumably by the RET1 TUTase.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.