ArticleRNA (New York, N.Y.)2005
The N- and C-terminal RNA recognition motifs of splicing factor Prp24 have distinct functions in U6 RNA binding.
Article in RNA (New York, N.Y.), 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 28 citations in OpenAlex.
- RNA modifications and Prp24 coordinate Lsm2-8 binding dynamics during S. cerevisiae U6 snRNP assembly.The Journal of biological chemistry · 2025Article
- Transcriptomic Analysis of TwoFrontiers in microbiology · 2020Article
- Architecture of the U6 snRNP reveals specific recognition of 3'-end processed U6 snRNA.Nature communications · 2018Article
- The life of U6 small nuclear RNA, from cradle to grave.RNA (New York, N.Y.) · 2018Review
- Structural requirements for protein-catalyzed annealing of U4 and U6 RNAs during di-snRNP assembly.Nucleic acids research · 2016Article
- Rapid isolation and single-molecule analysis of ribonucleoproteins from cell lysate by SNAP-SiMPull.RNA (New York, N.Y.) · 2015Article
- Core structure of the U6 small nuclear ribonucleoprotein at 1.7-Å resolution.Nature structural & molecular biology · 2014Article
- Deep mutational scanning of an RRM domain of the Saccharomyces cerevisiae poly(A)-binding protein.RNA (New York, N.Y.) · 2013Article
- Functional roles of protein splicing factors.Bioscience reports · 2012Review
- A novel occluded RNA recognition motif in Prp24 unwinds the U6 RNA internal stem loop.Nucleic acids research · 2011Article
- Structure and functional implications of a complex containing a segment of U6 RNA bound by a domain of Prp24.RNA (New York, N.Y.) · 2010Article
- (1)H, (13)C and (15)N resonance assignments of a ribonucleoprotein complex consisting of Prp24-RRM2 bound to a fragment of U6 RNA.Biomolecular NMR assignments · 2009Article
- Minimum-energy path for a u6 RNA conformational change involving protonation, base-pair rearrangement and base flipping.Journal of molecular biology · 2009Article
- Structure of yeast U6 snRNPs: arrangement of Prp24p and the LSm complex as revealed by electron microscopy.RNA (New York, N.Y.) · 2008Article
- A dynamic bulge in the U6 RNA internal stem-loop functions in spliceosome assembly and activation.RNA (New York, N.Y.) · 2007Article
- Network of coregulated spliceosome components revealed by zebrafish mutant in recycling factor p110.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Structure and interactions of the first three RNA recognition motifs of splicing factor prp24.Journal of molecular biology · 2007Article
- Resonance assignments for the two N-terminal RNA recognition motifs (RRM) of the S. cerevisiae pre-mRNA processing protein Prp24.Journal of biomolecular NMR · 2006Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Prp24 is an essential yeast U6 snRNP protein with four RNA recognition motifs (RRMs) that facilitates the association of U4 and U6 snRNPs during spliceosome assembly. Genetic interactions led to the proposal that RRMs 2 and 3 of Prp24 bind U6 RNA, while RRMs 1 and 4 bind U4 RNA. However, the function of each RRM has yet to be established through biochemical means. We compared the binding of recombinant full-length Prp24 and truncated forms lacking RRM 1 or RRM 4 with U6 RNA. Contrary to expectations, we found that the N-terminal segment containing RRM 1 is important for high-affinity binding to U6 RNA and for discrimination between wild-type U6 RNA and U6 with point mutations in the 3' intramolecular stem-loop. In contrast, deletion of RRM 4 and the C terminus did not significantly alter the affinity for U6 RNA, but resulted in the formation of higher order Prp24.U6 complexes. Truncation and internal deletion of U6 RNA mapped three Prp24-binding sites, with the central site providing most of the affinity for Prp24. A newly identified temperature-sensitive lethal point mutation in RRM 1 is exacerbated by mutations in the U6 RNA telestem, as is a mutation in RRM 2, but not one in RRM 3. We propose that RRMs 1 and 2 of yeast Prp24 bind the same central site in U6 RNA that is bound by the two RRMs of human Prp24, and that RRMs 3 and 4 bind lower affinity flanking sites, thereby restricting the stoichiometry of Prp24 binding.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.