ArticleEnvironmental health perspectives2005
Xenoestrogens at picomolar to nanomolar concentrations trigger membrane estrogen receptor-alpha-mediated Ca2+ fluxes and prolactin release in GH3/B6 pituitary tumor cells.
Article in Environmental health perspectives, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 125 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
125 citing papers in PubMed, 354 citations in OpenAlex.
- Advances in Research on Models of Oligoasthenozoospermia.Reproductive sciences (Thousand Oaks, Calif.) · 2026Review
- The Emerging Role of Prolactin as a Biomarker for Reproduction, Health, and Welfare in Wildlife Species: Elephants as a Model.Advances in experimental medicine and biology · 2026Review
- 17β-Trenbolone Increases the Release of Lipocalin 2 via the Brain-Liver Axis and Causes Alzheimer's Disease-Like Symptoms in CSDS-Induced Mice.Molecular neurobiology · 2025Article
- Reproductive Effects of Endocrine Disruptors in Domestic Ruminants: Integrating In Vitro and In Vivo Evidence.Animals : an open access journal from MDPI · 2025Review
- Endocrine-disrupting chemicals as prostate carcinogens.Nature reviews. Urology · 2025Review
- The impact, mechanisms and prevention strategies of environmental endocrine disruptors on male reproductive health.Frontiers in endocrinology · 2025Review
- Effect of Endocrine Disruptors on Testicular Function.Advances in experimental medicine and biology · 2025Review
- A systematic review of pesticide exposure, associated risks, and long-term human health impacts.Toxicology reports · 2024Review
- Effects of Endocrine-Disrupting Chemicals on Bone Health.Endocrinology and metabolism (Seoul, Korea) · 2024Review
- Multi- and transcriptomic changes of chronic exposure to bisphenol A reveals reproductive toxicity in male zebrafish.Fish physiology and biochemistry · 2023Article
- Different types of bisphenols alter ovarian steroidogenesis: Special attention to BPA.Heliyon · 2023Article
- Endocrine Disruptor Chemicals and Children's Health.International journal of molecular sciences · 2023Review
- Ancestral BPA exposure caused defects in the liver of medaka for four generations.The Science of the total environment · 2023Article
- Bisphenol A substitutes and obesity: a review of the epidemiology and pathophysiology.Frontiers in endocrinology · 2023Review
- Effects of Organochlorine Pesticide Residues in Maternal Body on Infants.Frontiers in endocrinology · 2022Review
- The Impact of Endocrine-Disrupting Chemicals in Male Fertility: Focus on the Action of Obesogens.Journal of xenobiotics · 2021Review
- Effects of Bisphenol A Released From Composite Fillings on Reproductive Hormone Levels in Men.International dental journal · 2021Article
- Bisphenol A and Metabolites in Meat and Meat Products: Occurrence, Toxicity, and Recent Development in Analytical Methods.Foods (Basel, Switzerland) · 2021Review
- Fighting Bisphenol A-Induced Male Infertility: The Power of Antioxidants.Antioxidants (Basel, Switzerland) · 2021Review
- Involvement of the Endocrine-Disrupting Chemical Bisphenol A (BPA) in Human Placentation.Journal of clinical medicine · 2020Article
65 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Xenoestrogens (XEs) are widespread in our environment and are known to have deleterious effects in animal (and perhaps human) populations. Acting as inappropriate estrogens, XEs are thought to interfere with endogenous estrogens such as estradiol (E2) to disrupt normal estrogenic signaling. We investigated the effects of E2 versus several XEs representing organochlorine pesticides (dieldrin, endosulfan, o',p'-dichlorodiphenylethylene), plastics manufacturing by-products/detergents (nonylphenol, bisphenol A), a phytoestrogen (coumestrol), and a synthetic estrogen (diethylstilbestrol) on the pituitary tumor cell subline GH3/B6/F10, previously selected for expression of high levels of membrane estrogen receptor-alpha. Picomolar to nanomolar concentrations of both E2 and XEs caused intracellular Ca2+ changes within 30 sec of administration. Each XE produced a unique temporal pattern of Ca2+ elevation. Removing Ca2+ from the extracellular solution abolished both spontaneous and XE-induced intracellular Ca2+ changes, as did 10 microM nifedipine. This suggests that XEs mediate their actions via voltage-dependent L-type Ca2+ channels in the plasma membrane. None of the Ca2+ fluxes came from intracellular Ca2+ stores. E2 and each XE also caused unique time- and concentration-dependent patterns of prolactin (PRL) secretion that were largely complete within 3 min of administration. PRL secretion was also blocked by nifedipine, demonstrating a correlation between Ca2+ influx and PRL secretion. These data indicate that at very low concentrations, XEs mediate membrane-initiated intracellular CCa2+ increases resulting in PRL secretion via a mechanism similar to that for E2, but with distinct patterns and potencies that could explain their abilities to disrupt endocrine functions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.