ArticleThe Biochemical journal2005
Cloning of ABCA17, a novel rodent sperm-specific ABC (ATP-binding cassette) transporter that regulates intracellular lipid metabolism.
Article in The Biochemical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 32 citations in OpenAlex.
- The human ATP-binding cassette (ABC) transporter superfamily.Human mutation · 2022Review
- AtABCA9 transporter supplies fatty acids for lipid synthesis to the endoplasmic reticulum.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- ATP-binding cassette transporter G2 activity in the bovine spermatozoa is modulated along the epididymal duct and at ejaculation.Biology of reproduction · 2012Article
- Drug transporters, the blood-testis barrier, and spermatogenesis.The Journal of endocrinology · 2011Review
- Epididymis cholesterol homeostasis and sperm fertilizing ability.Asian journal of andrology · 2011Review
- ATP-binding cassette transporters ABCA1, ABCA7, and ABCG1 in mouse spermatozoa.Biochemical and biophysical research communications · 2008Article
- The human ABC transporter pseudogene family: Evidence for transcription and gene-pseudogene interference.BMC genomics · 2008Article
- The ABCA subfamily--gene and protein structures, functions and associated hereditary diseases.Pflugers Archiv : European journal of physiology · 2007Review
- The human ortholog of the rodent testis-specific ABC transporter Abca17 is a ubiquitously expressed pseudogene (ABCA17P) and shares a common 5' end with ABCA3.BMC molecular biology · 2006Article
- New insights into the regulation of cholesterol efflux from the sperm membrane.Asian journal of andrologyReview
- Correlation of Adiponectin mRNA Abundance and Its Receptors with Quantitative Parameters of Sperm Motility in Rams.International journal of fertility & sterilityArticle
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The A subclass of the ABC (ATP-binding cassette) transporter superfamily has a structural feature that distinguishes it from other ABC transporters, and is proposed to be involved in the transmembrane transport of endogenous lipids. Here we have cloned mouse and rat full-length cDNAs of ABCA17, a novel ABC transporter belonging to the A subclass. Mouse and rat ABCA17 proteins comprise 1733 and 1773 amino acid residues respectively, having 87.3% amino acid identity; mouse ABCA17 has amino acid identities of 55.3% and 36.7% with mouse ABCA3 and sea urchin ABCA respectively. RNA blot and quantitative real-time PCR analyses showed that ABCA17 mRNA is expressed exclusively in the testis. Examination of testis by in situ hybridization showed that ABCA17 mRNA is expressed in germ cells, mainly spermatocytes, in the seminiferous tubule. Immunoblot analysis using a specific antibody showed that ABCA17 is a protein of 200 kDa, and immunohistochemical analysis demonstrated that the protein is detected in the anterior head of sperm and elongated spermatids. ABCA17 was localized in the endoplasmic reticulum in transiently transfected HEK293 cells. Metabolic labelling analysis showed that intracellular esterified lipids, including cholesteryl esters, fatty acid esters and triacylglycerols, were significantly decreased in HEK293 cells stably expressing ABCA17 compared with untransfected cells. These results suggest that ABCA17 may play a role in regulating lipid composition in sperm.
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