ArticlePlanta2005
Responsive modes of Medicago sativa proline dehydrogenase genes during salt stress and recovery dictate free proline accumulation.
Article in Planta, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 78 citations in OpenAlex.
- Proline: A Reliable Biochemical Marker of Plant Abiotic Stress Tolerance?Plants (Basel, Switzerland) · 2026Review
- Proline Accumulation in Barley Under Salinity Is ABA-Independent, but Relies on the Level of Oxidative Stress When Modulated by Mo and W Ions.International journal of molecular sciences · 2026Article
- The nonphototrophic hypocotyl 3 (NPH3) domain protein NRL5 is a trafficking-associated GTPase essential for drought resistance.Science advances · 2024Article
- Burning questions for a warming and changing world: 15 unknowns in plant abiotic stress.The Plant cell · 2023Article
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- ASCORBATE PEROXIDASE6 delays the onset of age-dependent leaf senescence.Plant physiology · 2021Article
- How Does Proline Treatment Promote Salt Stress Tolerance During Crop Plant Development?Frontiers in plant science · 2020Review
- Article
- Article
- Comparative analysis of alfalfa (Medicago sativa L.) leaf transcriptomes reveals genotype-specific salt tolerance mechanisms.BMC plant biology · 2018Article
- Proline Coordination with Fatty Acid Synthesis and Redox Metabolism of Chloroplast and Mitochondria.Plant physiology · 2016Article
- Water deficit stress-induced changes in carbon and nitrogen partitioning in Chenopodium quinoa Willd.Planta · 2016Article
- Proline accumulation and metabolism-related genes expression profiles in Kosteletzkya virginica seedlings under salt stress.Frontiers in plant science · 2015Article
- Dynamic proline metabolism: importance and regulation in water limited environments.Frontiers in plant science · 2015Article
- Antioxidant response and related gene expression in aged oat seed.Frontiers in plant science · 2015Article
- Plastid osmotic stress activates cellular stress responses in Arabidopsis.Plant physiology · 2014Article
- Proline and its metabolism enzymes in cucumber cell cultures during acclimation to salinity.Protoplasma · 2014Article
- The accumulation of endogenous proline induces changes in gene expression of several antioxidant enzymes in leaves of transgenic Swingle citrumelo.Molecular biology reports · 2013Article
- Salt stress encourages proline accumulation by regulating proline biosynthesis and degradation in Jerusalem artichoke plantlets.PloS one · 2013Article
- Essential role of tissue-specific proline synthesis and catabolism in growth and redox balance at low water potential.Plant physiology · 2011Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Free proline accumulation is an innate response of many plants to osmotic stress. To characterize transcriptional regulation of the key proline cycle enzymes in alfalfa (Medicago sativa), two proline dehydrogenase (MsPDH) genes and a partial sequence of Delta (1) -pyrroline-5-carboxylate dehydrogenase (MsP5CDH) gene were identified and cloned. The two MsPDH genes share a high nucleotide sequence homology and a similar exon/intron structure. Estimation of transcript levels during salt stress and recovery revealed that proline accumulation during stress was linearly correlated with a strong decline in MsPDH transcript levels, while Delta (1) -pyrroline-5-carboxylate synthetase (MsP5CS) and MsP5CDH steady-state transcript levels remained essentially unchanged. MsPDH transcript levels dramatically decreased in a fast, salt concentration-dependent manner. The extent of salt-induced proline accumulation also correlated with salt concentrations. Salt-induced repression of MsPDH1 promoter linked to the GUS reporter gene confirmed that the decline in MsPDH transcript levels was due to less transcription initiation. Contrary to the salt-dependent repression, a rapid induction of MsPDH transcription occurred at a very early stage of the recovery process, independently of earlier salt treatments. Hence our results suggest the existence of two different regulatory modes of MsPDH expression; the repressing mode that quantifies salt concentration in an as yet unknown mechanism and the "rehydration"-enhancing mode that responds to stress relief in a maximal induction of MsPDH transcription. As yet the components of salt sensing as well as those that might interact with MsPDH promoter to reduce transcription are still unknown.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.