ArticleProceedings of the National Academy of Sciences of the United States of America2005
Molecular organization of the Ndc80 complex, an essential kinetochore component.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 167 papers.
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Who cites it
167 citing papers in PubMed, 278 citations in OpenAlex.
- Structure and function of the outer kinetochore.Nature reviews. Molecular cell biology · 2026Review
- Kinetochore-microtubule attachments are strengthened by Cnn1 stabilization of Stu2.bioRxiv : the preprint server for biology · 2026Article
- Assembly and phosphoregulatory mechanisms of the budding yeast outer kinetochore KMN complex.The Journal of cell biology · 2026Article
- Microtubule end stabilisation by cooperative oligomers of Ska and Ndc80 complexes.The EMBO journal · 2026Article
- CENcyclopedia: dynamic landscape of kinetochore architecture throughout the cell cycle.Nature communications · 2025Article
- Article
- Force transmission through the inner kinetochore is enhanced by centromeric DNA sequences.eLife · 2025Article
- A role for the kinetochore protein, NUF2, in ribosome biogenesis.Molecular biology of the cell · 2025Article
- Kinetochores grip microtubules with directionally asymmetric strength.The Journal of cell biology · 2025Article
- The multifaceted functions of SPC25 in cancer: from molecular pathways to targeted therapy.Frontiers in medicine · 2025Review
- Analysis of a cancer-associated mutation in the budding yeast Nuf2 kinetochore protein.microPublication biology · 2025Article
- Architecture of native kinetochores revealed by structural studies utilizing a thermophilic yeast.Current biology : CB · 2024Article
- Disruption of Plasmodium falciparum kinetochore proteins destabilises the nexus between the centrosome equivalent and the mitotic apparatus.Nature communications · 2024Article
- A conserved site on Ndc80 complex facilitates dynamic recruitment of Mps1 to yeast kinetochores to promote accurate chromosome segregation.Current biology : CB · 2024Article
- A communication hub for phosphoregulation of kinetochore-microtubule attachment.Current biology : CB · 2024Article
- Structure of the human KMN complex and implications for regulation of its assembly.Nature structural & molecular biology · 2024Article
- Architecture and flexibility of native kinetochores revealed by structural studies utilizing a thermophilic yeast.bioRxiv : the preprint server for biology · 2024Article
- A role for β-1,6- and β-1,3-glucans in kinetochore function in Saccharomyces cerevisiae.Genetics · 2024Article
- Humanization reveals pervasive incompatibility of yeast and human kinetochore components.G3 (Bethesda, Md.) · 2023Article
- An interaction hub on Ndc80 complex facilitates dynamic recruitment of Mps1 to yeast kinetochores to promote accurate chromosome segregation.bioRxiv : the preprint server for biology · 2023Article
107 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The four-protein Ndc80 complex, an essential kinetochore component conserved from yeast to humans, plays an indispensable role in proper chromosome alignment and segregation during mitosis. In higher eukaryotes, the homologous complex probably resides in the middle domain of the trilaminar kinetochore, linking centromeric heterochromatin with microtubule-associated structures. We have prepared recombinant Ndc80 complex by pairwise coexpression of its components (Ndc80p and Nuf2p; Spc24p and Spc25p) and shown that they form independently stable subcomplexes. Rotary shadowing electron microscopy, combined with limited proteolysis and antibody labeling, demonstrates that the heterotetrameric Ndc80 complex is an approximately 570-A-long rod, with globular regions at either end. The shaft contains alpha-helical coiled-coil segments from each of the two subcomplexes, linked end-to-end. When integrated with published observations derived from inactivating the components of Ndc80, the molecular organization we deduce suggests that the Spc24p/Spc25p end of the rod faces the centromere and the Ndc80p/Nuf2p end faces a spindle microtubule.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.