ArticleArchaea (Vancouver, B.C.)2002
Functional organization of a single nif cluster in the mesophilic archaeon Methanosarcina mazei strain Gö1.
Article in Archaea (Vancouver, B.C.), 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 46 citations in OpenAlex.
- Article
- ModE Regulates Alternative Nitrogenase Expression in the Methanogen Methanosarcina acetivorans.Molecular microbiology · 2025Article
- Quantitative analysis of amino acid excretion by Methanothermobacter marburgensis under NScientific reports · 2025Article
- Exploring diazotrophic diversity: unveiling Nif core distribution and evolutionary patterns in nitrogen-fixing organisms.BMC genomics · 2025Article
- Functional insights into Solo-Cas4 inmicroLife · 2025Article
- Guided tour through the small protein landscape ofmicroLife · 2025Article
- Nitrogen fixation by methanogenic Archaea, literature review and DNA database-based analysis; significance in face of climate change.Archives of microbiology · 2024Review
- Uncovering the small proteome of Methanosarcina mazei using Ribo-seq and peptidomics under different nitrogen conditions.Nature communications · 2024Article
- Expression of V-nitrogenase and Fe-nitrogenase inApplied and environmental microbiology · 2023Article
- Active in vivo translocation of the Methanosarcina mazei Gö1 Casposon.Nucleic acids research · 2023Article
- Newly Established Genetic System for Functional Analysis of MetSV.International journal of molecular sciences · 2023Article
- Article
- Article
- Cross-cleavage activity of Cas6b in crRNA processing of two different CRISPR-Cas systems in Methanosarcina mazei Gö1.RNA biology · 2019Article
- Evolution and Functional Analysis ofInternational journal of molecular sciences · 2019Article
- Methanosarcina Spherical Virus, a Novel Archaeal Lytic Virus Targeting Methanosarcina Strains.Journal of virology · 2017Article
- sRNARNA biology · 2017Article
- Small regulatory RNAs in Archaea.RNA biology · 2014Review
- Distribution of nitrogen fixation and nitrogenase-like sequences amongst microbial genomes.BMC genomics · 2012Article
- Establishing a markerless genetic exchange system for Methanosarcina mazei strain Gö1 for constructing chromosomal mutants of small RNA genes.Archaea (Vancouver, B.C.) · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The mesophilic methanogenic archaeon Methanosarcina mazei strain Gö1 is able to utilize molecular nitrogen (N2) as its sole nitrogen source. We have identified and characterized a single nitrogen fixation (nif) gene cluster in M. mazei Gö1 with an approximate length of 9 kbp. Sequence analysis revealed seven genes with sequence similarities to nifH, nifI1, nifI2, nifD, nifK, nifE and nifN, similar to other diazotrophic methanogens and certain bacteria such as Clostridium acetobutylicum, with the two glnB-like genes (nifI1 and nifI2) located between nifH and nifD. Phylogenetic analysis of deduced amino acid sequences for the nitrogenase structural genes of M. mazei Gö1 showed that they are most closely related to Methanosarcina barkeri nif2 genes, and also closely resemble those for the corresponding nif products of the gram-positive bacterium C. acetobutylicum. Northern blot analysis and reverse transcription PCR analysis demonstrated that the M. mazei nif genes constitute an operon transcribed only under nitrogen starvation as a single 8 kb transcript. Sequence analysis revealed a palindromic sequence at the transcriptional start site in front of the M. mazei nifH gene, which may have a function in transcriptional regulation of the nif operon.
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