Evidence map›Paper›PMID 15803142›Full record

ArticleCancer gene therapy2005

Effect of EBV LMP1 targeted DNAzymes on cell proliferation and apoptosis.

Zhong-Xin Lu, Mao Ye, Guang-Rong Yan, Qun Li, Min Tang, Leo M Lee, Lun-Quan Sun, Ya Cao

Registry-linked trialAbstract read
PubMed Publisher
In one paragraph

Article in Cancer gene therapy, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT01449942 (Phase I/II Study of EBV-LMP1 Targeted DNAzyme in Nasopharyngeal Carcinoma), which is not on this map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
2.3field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT01449942 phase1 / phase2completednot on this mapstarted 2009, after this paper: background citation

Phase I/II Study of EBV-LMP1 Targeted DNAzyme in Nasopharyngeal Carcinoma

TypeinterventionalSponsorXiangya Hospital of Central South UniversityRan2009 to 2011Enrolled40ConditionsNasopharyngeal CarcinomaArmsDNAzyme targeting EBV-LMP1 (DZ1), Saline
3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 69 citations in OpenAlex.

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  13. Dysfunction of mitochondria due to environmental carcinogens in nasopharyngeal carcinoma in the ethnic group of Northeast Indian population.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2014
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 3 countries.

Zhong-Xin LuCancer Research Institute, Xiangya School of Medicine, Central South University, Changsha, China.
Mao Ye
Guang-Rong Yan
Qun Li
Min Tang
Leo M Lee
Lun-Quan Sun
Ya Cao
Central South University · CNScience Applications International Corporation (United States) · USSt Vincent's Clinic · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The latent membrane protein (LMP1) encoded by Epstein-Barr virus (EBV) has been suggested to be one of the major oncogenic factors in EBV-mediated carcinogenesis. RNA-cleaving DNA enzymes are catalytic nucleic acids that bind and cleave a target RNA in a highly sequence-specific manner. In this study, we explore the potential of using DNAzymes as a therapeutic approach to EBV-associated carcinomas by targeting the LMP1 gene. In all, 13 different phosphorothioate-modified "10-23" deoxyribozymes (DNAzymes) were designed and synthesized against the LMP1 mRNA and transfected into B95-8 cells, which constitutively express the LMP1. Fluorescence microscopy was used to examine the cellular uptake and distribution in B95-8 cells. As demonstrated in Western blots, three out of 13 deoxyribozymes significantly downregulated the expression of LMP1 in B95-8 cells. These DNAzymes were shown to markedly inhibit B95-8 cell growth compared with a disabled DNAzyme and untreated controls, as determined by an alamarBlue Assay. It was further demonstrated that these DNAzymes arrested the B95-8 cells in G0/G1 using flow cytometry. Interestingly, the active DNAzymes could also downregulate the expression of Bcl-2 gene in treated cells, suggesting a close association between the LMP1 and Bcl-2 genes and their involvement in apoptosis. This was further confirmed with the result that the DNAzymes could induce the release of cytochrome c from mitochondria, which is the hallmark of the apoptosis. The present results suggest that the LMP1 may present a potential target for DNAzymes towards the EBV-associated carcinoma through cell proliferation and apoptosis pathways.

Indexed as

ApoptosisCell ProliferationAnimalsBlotting, WesternCell Line, TransformedCytochromes cDNA, CatalyticDNA, Single-StrandedHerpesvirus 4, HumanMicroscopy, FluorescenceMitochondriaProto-Oncogene Proteins c-bcl-2RNA, MessengerViral Matrix ProteinsCytochromes cDNA, CatalyticDNA, Single-StrandedEBV-associated membrane antigen, Epstein-Barr virusProto-Oncogene Proteins c-bcl-2RNA-cleaving DNA 10-23RNA, MessengerViral Matrix Proteins

Identifiers

PMID15803142
OpenAlexW1967217444

What OpenQuestion holds

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Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.