ArticleGenetics2005
Chromosome sorting in tetraploid wheat and its potential for genome analysis.
Article in Genetics, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
41 citing papers in PubMed.
- Subtle but Significant: Intraspecific Genome Size Variation in Durum Wheat Landraces and Cultivars.Plants (Basel, Switzerland) · 2026Article
- Current status and future perspective of genomics-assisted breeding in wheat (Breeding science · 2026Article
- Review
- A linkage map of Aegilops biuncialis reveals significant genomic rearrangements compared to bread wheat.The plant genome · 2025Article
- Advanced environmental scanning electron microscopy reveals natural surface nano-morphology of condensed mitotic chromosomes in their native state.Scientific reports · 2024Article
- Dissection of a rapidly evolving wheat resistance gene cluster by long-read genome sequencing accelerated the cloning of Pm69.Plant communications · 2024Article
- Aegilops sharonensis genome-assisted identification of stem rust resistance gene Sr62.Nature communications · 2022Article
- Flow karyotyping of wheat-Frontiers in plant science · 2022Article
- Chromosome analysis and sorting.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2021Review
- Development of DNA Markers From Physically Mapped Loci inFrontiers in plant science · 2021Article
- FISH karyotype comparison between AJournal of applied genetics · 2020Article
- Dissecting the Complex Genome of Crested Wheatgrass by Chromosome Flow Sorting.The plant genome · 2019Article
- Development of an oligonucleotide dye solution facilitates high throughput and cost-efficient chromosome identification in peanut.Plant methods · 2019Article
- MicroRNAs in durum wheat seedlings under chronic and short-term nitrogen stress.Functional & integrative genomics · 2018Article
- Evolution of the S-Genomes inFrontiers in plant science · 2018Article
- Cytomolecular discrimination of the AJournal of applied genetics · 2017Article
- Molecular cytogenetic characterization of novel wheat-Thinopyrum bessarabicum recombinant lines carrying intercalary translocations.Chromosoma · 2016Article
- The utility of flow sorting to identify chromosomes carrying a single copy transgene in wheat.Plant methods · 2016Article
- Intraspecific Polymorphisms of Cytogenetic Markers Mapped on Chromosomes of Triticum polonicum L.PloS one · 2016Article
- Flow sorting of C-genome chromosomes from wild relatives of wheat Aegilops markgrafii, Ae. triuncialis and Ae. cylindrica, and their molecular organization.Annals of botany · 2015Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study evaluates the potential of flow cytometry for chromosome sorting in durum wheat (Triticum turgidum Desf. var. durum, 2n = 4x = 28). Histograms of fluorescence intensity (flow karyotypes) obtained after the analysis of DAPI-stained chromosomes consisted of three peaks. Of these, one represented chromosome 3B, a small peak corresponded to chromosomes 1A and 6A, and a large peak represented the remaining 11 chromosomes. Chromosomes sorted onto microscope slides were identified after fluorescence in situ hybridization (FISH) with probes for GAA microsatellite, pSc119.2, and Afa repeats. Genomic distribution of these sequences was determined for the first time in durum wheat and a molecular karyotype has been developed for this crop. Flow karyotyping in double-ditelosomic lines of durum wheat revealed that the lines facilitated sorting of any arm of the wheat A- and B-genome chromosomes. Compared to hexaploid wheat, flow karyotype of durum wheat is less complex. This property results in better discrimination of telosomes and high purities in sorted fractions, ranging from 90 to 98%. We have demonstrated that large insert libraries can be created from DNA purified using flow cytometry. This study considerably expands the potential of flow cytogenetics for use in wheat genomics and opens the possibility of sequencing the genome of this important crop one chromosome arm at a time.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.