ArticleBiotechnology and bioengineering2005
Gene delivery through cell culture substrate adsorbed DNA complexes.
Article in Biotechnology and bioengineering, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
61 citing papers in PubMed.
- Injectable GSK3 inhibitor (Tideglusib) hydrogel versus enhanced β-tricalcium phosphate in tibial bone healing (in vitro and in vivo study).Odontology · 2026Article
- Advances in viral vector-based delivery systems for gene therapy: a comprehensive review.3 Biotech · 2025Review
- Evaluation of adsorption of DNA/PEI polyplexes to tubing materials.European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V · 2022Article
- Topography-Mediated Enhancement of Nonviral Gene Delivery in Stem Cells.Pharmaceutics · 2022Article
- A Comprehensive Review on the Applications of Exosomes and Liposomes in Regenerative Medicine and Tissue Engineering.Polymers · 2021Review
- Bioactivity Comparison of Electrospun PCL Mats and Liver Extracellular Matrix as Scaffolds for HepG2 Cells.Polymers · 2021Article
- Wound Healing Activity of Nanoclay/Spring Water Hydrogels.Pharmaceutics · 2020Article
- Screening a chemically defined extracellular matrix mimetic substrate library to identify substrates that enhance substrate-mediated transfection.Experimental biology and medicine (Maywood, N.J.) · 2020Article
- Biomaterial substrate modifications that influence cell-material interactions to prime cellular responses to nonviral gene delivery.Experimental biology and medicine (Maywood, N.J.) · 2019Review
- Free Polyethylenimine Enhances Substrate-Mediated Gene Delivery on Titanium Substrates Modified With RGD-Functionalized Poly(acrylic acid) Brushes.Frontiers in chemistry · 2019Article
- ECM turnover-stimulated gene delivery through collagen-mimetic peptide-plasmid integration in collagen.Acta biomaterialia · 2017Article
- Poly(lactic acid) nanofibrous scaffolds for tissue engineering.Advanced drug delivery reviews · 2016Review
- Integration of growth factor gene delivery with collagen-triggered wound repair cascades using collagen-mimetic peptides.Bioengineering & translational medicine · 2016Article
- Controlling the surface-mediated release of DNA using 'mixed multilayers'.Bioengineering & translational medicine · 2016Article
- Polymer Multilayers that Promote the Rapid Release and Contact Transfer of DNA.Biomacromolecules · 2015Article
- Nanoparticle-based bioactive agent release systems for bone and cartilage tissue engineering.Regenerative therapy · 2015Review
- Liposomes in tissue engineering and regenerative medicine.Journal of the Royal Society, Interface · 2014Review
- Electrospun nanofibers as versatile interfaces for efficient gene delivery.Journal of biological engineering · 2014Review
- Surface-mediated nucleic acid delivery by lipoplexes prepared in microwell arrays.Small (Weinheim an der Bergstrasse, Germany) · 2013Article
- Nanotopography-mediated reverse uptake for siRNA delivery into neural stem cells to enhance neuronal differentiation.Scientific reports · 2013Article
1 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Efficient gene delivery is a fundamental goal of biotechnology and has numerous applications in both basic and applied science. Substrate-mediated delivery and reverse transfection enhance gene transfer by increasing the concentration of DNA in the cellular microenvironment through immobilizing a plasmid to a cell culture substrate prior to cell seeding. In this report, we examine gene delivery of plasmids that were complexed with cationic polymers (polyplexes) or lipids (lipoplexes) and subsequently immobilized to cell culture or biomaterial substrates by adsorption. Polyplexes and lipoplexes were adsorbed to either tissue culture polystyrene or serum-adsorbed tissue culture polystyrene. The quantity of DNA immobilized increased with time of exposure, and the deposition rate and final amount deposited depended upon the properties of the substrate and complex. For polyplexes, serum modification enhanced reporter gene expression up to 1500-fold relative to unmodified substrates and yielded equivalent or greater expression compared to bolus delivery. For lipoplexes, serum modification significantly increased the number of transfected cells relative to unmodified substrates yet provided similar levels of expression. Immobilized complexes transfect primary cells with improved cellular viability relative to bolus delivery. Finally, this substrate-mediated delivery approach was extended to a widely used biomaterial, poly(lactide-co-glycolide). Immobilization of DNA complexes to tissue culture polystyrene substrates can be a useful tool for enhancing gene delivery for in vitro studies. Additionally, adapting this system to biomaterials may facilitate application to fields such as tissue engineering.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.