ArticleGene therapy2005
Detection of plasmid DNA vectors following gene transfer to the murine airways.
Article in Gene therapy, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 41 citations in OpenAlex.
- Contribution of Epigenetic Modifications to the Decline in Transgene Expression from Plasmid DNA in Mouse Liver.Pharmaceutics · 2015Article
- Toxicology study assessing efficacy and safety of repeated administration of lipid/DNA complexes to mouse lung.Gene therapy · 2014Article
- Gene delivery potential of biofunctional carbonate apatite nanoparticles in lungs.BioMed research international · 2014Article
- Article
- Rapid identification of novel functional promoters for gene therapy.Journal of molecular medicine (Berlin, Germany) · 2012Article
- A designed curved DNA sequence remarkably enhances transgene expression from plasmid DNA in mouse liver.Gene therapy · 2012Article
- Irradiation, cisplatin, and 5-azacytidine upregulate cytomegalovirus promoter in tumors and muscles: implementation of non-invasive fluorescence imaging.Molecular imaging and biology · 2011Article
- pEPito: a significantly improved non-viral episomal expression vector for mammalian cells.BMC biotechnology · 2010Article
- Developing cell therapy techniques for respiratory disease: intratracheal delivery of genetically engineered stem cells in a murine model of airway injury.Human gene therapy · 2009Article
- Lentiviral transduction of the murine lung provides efficient pseudotype and developmental stage-dependent cell-specific transgene expression.Gene therapy · 2008Article
- BHLHB2 controls Bdnf promoter 4 activity and neuronal excitability.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- Transient activation of transgene expression by hydrodynamics-based injection may cause rapid decrease in plasmid DNA expression.Gene therapy · 2007Article
- Electroporation-mediated transfer of plasmids to the lung results in reduced TLR9 signaling and inflammation.Gene therapy · 2007Article
- Long-term persistence of gene expression from adeno-associated virus serotype 5 in the mouse airways.Gene therapy · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Non-viral gene therapy is being considered as a treatment for cystic fibrosis. In clinical studies and in studies using the mouse airways as a model, current formulations result in only transient transgene expression. A number of reasons for this have been proposed including the loss of plasmid DNA from cells. The aim of these studies was to investigate why transgene expression from non-viral vectors is transient in the mouse lung. Plasmid DNA encoding the luciferase reporter gene was complexed with the cationic lipid GL67 and delivered to the mouse airways. The persistence of plasmid DNA in the mouse lungs was investigated using quantitative PCR and Southern hybridization. Results showed that intact plasmid DNA persisted in the mouse lung in the absence of any detectable luciferase activity. The de novo methylation of plasmid DNA in vivo was investigated as a potential cause of this transient gene expression but results suggested that plasmid DNA does not become de novo methylated in the mouse lung. Therefore processes other than the loss of plasmid DNA from the lung or the de novo methylation of plasmid DNA vectors must be responsible for the transient transgene expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.