ArticleNucleic acids research2005
Efficient and seamless DNA recombineering using a thymidylate synthase A selection system in Escherichia coli.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
46 citing papers in PubMed, 104 citations in OpenAlex.
- Seamless site-directed mutagenesis in complex cloned DNA sequences using the RedEx method.Nature protocols · 2024Review
- Genetically Engineered Microorganisms and Their Impact on Human Health.International journal of clinical practice · 2024Review
- Recombineering: Genetic Engineering in Escherichia coli Using Homologous Recombination.Current protocols · 2023Article
- Characterization of ColE1 Production for RobustACS synthetic biology · 2022Article
- Review
- Article
- Intein Inhibitors as Novel Antimicrobials: Protein Splicing in Human Pathogens, Screening Methods, and Off-Target Considerations.Frontiers in molecular biosciences · 2021Review
- Creation of Golden Gate constructs for gene doctoring.BMC biotechnology · 2020Article
- Development of a Method for Simultaneous Generation of Multiple Genetic Modification inFrontiers in genetics · 2020Article
- Bacterial Genetic Engineering by Means of Recombineering for Reverse Genetics.Frontiers in microbiology · 2020Review
- The prokaryotic Argonaute proteins enhance homology sequence-directed recombination in bacteria.Nucleic acids research · 2019Article
- Article
- Characterization of Inducible ccdB Gene as a Counterselectable Marker in Escherichia coli Recombineering.Current microbiology · 2017Article
- Oligo- and dsDNA-mediated genome editing using a tetA dual selection system in Escherichia coli.PloS one · 2017Article
- Synthetic biology approaches to biological containment: pre-emptively tackling potential risks.Essays in biochemistry · 2016Review
- λ Recombination and Recombineering.EcoSal Plus · 2016Review
- REPLACR-mutagenesis, a one-step method for site-directed mutagenesis by recombineering.Scientific reports · 2016Article
- PCR-Based Seamless Genome Editing with High Efficiency and Fidelity in Escherichia coli.PloS one · 2016Article
- A set of powerful negative selection systems for unmodified Enterobacteriaceae.Nucleic acids research · 2015Article
- Repetitive genomic insertion of gene-sized dsDNAs by targeting the promoter region of a counter-selectable marker.Scientific reports · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lambda-red system-based recombinogenic engineering is a powerful new method to engineer DNA without the need for restriction enzymes or ligases. Here, we report the use of a single selectable marker to enhance the usefulness of this approach. The strategy is to utilize the thymidylate synthase A (thyA) gene, which encodes an enzyme involved in the synthesis of thymidine 5'-triphosphate, for both positive and negative selection. With this approach, we successfully created point mutations in plasmid and bacterial artificial chromosome (BAC) DNA containing the mouse Col10a1 gene. The results showed that the thyA selection system is highly efficient and accurate, giving an average of >90% selection efficiency. This selection system produces DNA that is free from permanent integration of unwanted sequences, thus allowing unlimited rounds of modifications if required.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.