ArticleMolecular and cellular biology2005
Identification of a novel, intraperoxisomal pex14-binding site in pex13: association of pex13 with the docking complex is essential for peroxisomal matrix protein import.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- The peroxisome protein translocation machinery is developmentally regulated in the fungusMicrobiology spectrum · 2024Article
- Protein import into peroxisomes occurs through a nuclear pore-like phase.Science (New York, N.Y.) · 2022Article
- Glycosome heterogeneity in kinetoplastids.Biochemical Society transactions · 2021Review
- MrPEX33 is involved in infection-related morphogenesis and pathogenicity of Metarhizium robertsii.Applied microbiology and biotechnology · 2021Article
- Pex14p Phosphorylation Modulates Import of Citrate Synthase 2 Into Peroxisomes inFrontiers in cell and developmental biology · 2020Article
- A defect in the peroxisomal biogenesis in germ cells induces a spermatogenic arrest at the round spermatid stage in mice.Scientific reports · 2019Article
- Hansenula polymorpha Aat2p is targeted to peroxisomes via a novel Pex20p-dependent pathway.FEBS letters · 2018Article
- Peroxisome Function, Biogenesis, and Dynamics in Plants.Plant physiology · 2018Review
- PEX14 binding to Arabidopsis PEX5 has differential effects on PTS1 and PTS2 cargo occupancy of the receptor.FEBS letters · 2014Article
- PEX5 and ubiquitin dynamics on mammalian peroxisome membranes.PLoS computational biology · 2014Article
- Import of proteins into the peroxisomal matrix.Frontiers in physiology · 2013Review
- Lipid droplets and peroxisomes: key players in cellular lipid homeostasis or a matter of fat--store 'em up or burn 'em down.Genetics · 2013Review
- Genetic dissection of peroxisome-associated matrix protein degradation in Arabidopsis thaliana.Genetics · 2013Article
- A simple recipe for the non-expert bioinformaticist for building experimentally-testable hypotheses for proteins with no known homologs.Journal of structural and functional genomics · 2012Review
- The peroxin loss-of-function mutation abstinence by mutual consent disrupts male-female gametophyte recognition.Current biology : CB · 2008Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The peroxisomal docking complex is a key component of the import machinery for matrix proteins. The core protein of this complex, Pex14, is thought to represent the initial docking site for the import receptors Pex5 and Pex7. Associated with this complex is a fraction of Pex13, another essential component of the import machinery. Here we demonstrate that Pex13 directly binds Pex14 not only via its SH3 domain but also via a novel intraperoxisomal site. Furthermore, we demonstrate that Pex5 also contributes to the association of Pex13 with Pex14. Peroxisome function was affected only mildly by mutations within the novel Pex14 interaction site of Pex13 or by the non-Pex13-interacting mutant Pex5(W204A). However, when these constructs were tested in combination, PTS1-dependent import and growth on oleic acid were severely compromised. When the SH3 domain-mediated interaction of Pex13 with Pex14 was blocked on top of that, PTS2-dependent matrix protein import was completely compromised and Pex13 was no longer copurified with the docking complex. We conclude that the association of Pex13 with Pex14 is an essential step in peroxisomal protein import that is enabled by two direct interactions and by one that is mediated by Pex5, a result which indicates a novel, receptor-independent function of Pex5.
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