ArticleJournal of virology2005
The p92 polymerase coding region contains an internal RNA element required at an early step in Tombusvirus genome replication.
Article in Journal of virology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
52 citing papers in PubMed, 95 citations in OpenAlex.
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- The non-template functions of helper virus RNAs create optimal replication conditions to enhance the proliferation of satellite RNAs.PLoS pathogens · 2024Article
- Race against Time between the Virus and Host: Actin-Assisted Rapid Biogenesis of Replication Organelles is Used by TBSV to Limit the Recruitment of Cellular Restriction Factors.Journal of virology · 2022Article
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- Role reversal of functional identity in host factors: Dissecting features affecting pro-viral versus antiviral functions of cellular DEAD-box helicases in tombusvirus replication.PLoS pathogens · 2020Article
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- A trans-activator-like structure in RCNMV RNA1 evokes the origin of the trans-activator in RNA2.PLoS pathogens · 2020Article
- Host protein chaperones, RNA helicases and the ubiquitin network highlight the arms race for resources between tombusviruses and their hosts.Advances in virus research · 2020Article
- Interviral Recombination between Plant, Insect, and Fungal RNA Viruses: Role of the Intracellular CaJournal of virology · 2019Article
- Blocking tombusvirus replication through the antiviral functions of DDX17-like RH30 DEAD-box helicase.PLoS pathogens · 2019Article
- Intragenomic Long-Distance RNA-RNA Interactions in Plus-Strand RNA Plant Viruses.Frontiers in microbiology · 2018Review
- Atypical RNA Elements Modulate Translational Readthrough in Tobacco Necrosis Virus D.Journal of virology · 2017Article
- RNA virus replication depends on enrichment of phosphatidylethanolamine at replication sites in subcellular membranes.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- The proteasomal Rpn11 metalloprotease suppresses tombusvirus RNA recombination and promotes viral replication via facilitating assembly of the viral replicase complex.Journal of virology · 2015Article
- Coordinated function of cellular DEAD-box helicases in suppression of viral RNA recombination and maintenance of viral genome integrity.PLoS pathogens · 2015Article
- Construction of a synthetic infectious cDNA clone of Grapevine Algerian latent virus (GALV-Nf) and its biological activity in Nicotiana benthamiana and grapevine plants.Virology journal · 2014Article
- The hop-like stress-induced protein 1 cochaperone is a novel cell-intrinsic restriction factor for mitochondrial tombusvirus replication.Journal of virology · 2014Article
- Template role of double-stranded RNA in tombusvirus replication.Journal of virology · 2014Article
- Article
- Inactivation of the host lipin gene accelerates RNA virus replication through viral exploitation of the expanded endoplasmic reticulum membrane.PLoS pathogens · 2014Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The replication of positive-strand RNA viral genomes involves various cis-acting RNA sequences. Generally, regulatory RNA sequences are present at or near genomic termini; however, internal replication elements (IREs) also exist. Here we report the structural and functional characterization of an IRE present in the readthrough portion of the p92 polymerase gene of Tomato bushy stunt virus. Analysis of this element in the context of a noncoding defective interfering RNA revealed a functional core structure composed of two noncontiguous segments of sequence that interact with each other to form an extended helical conformation. IRE activity required maintenance of several base-paired sections as well as two distinct structural features: (i) a short, highly conserved segment that can potentially form two different and mutually exclusive structures and (ii) an internal loop that contains a critical CC mismatch. The IRE was also shown to play an essential role within the context of the viral genome. In vivo analysis with novel RNA-based temperature-sensitive genomic mutants and translationally active subgenomic viral replicons revealed the following about the IRE: (i) it is active in the positive strand, (ii) it is dispensable late in the viral RNA replication process, and (iii) it is functionally inhibited by active translation over its sequence. Together, these results suggest that IRE activity is required in the cytosol at an early step in the viral replication process, such as template recruitment and/or replicase complex assembly.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.