ArticleNucleic acids research1992
cis-acting elements responsible for muscle-specific expression of the myosin heavy chain beta gene.
Article in Nucleic acids research, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 42 citations in OpenAlex.
- Differential regulation of the myosin heavy chain genes alpha and beta in rat atria and ventricles: role of antisense RNA.Thyroid : official journal of the American Thyroid Association · 2008Article
- Fgfr4 is required for effective muscle regeneration in vivo. Delineation of a MyoD-Tead2-Fgfr4 transcriptional pathway.The Journal of biological chemistry · 2006Article
- Organization of human and mouse skeletal myosin heavy chain gene clusters is highly conserved.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Tissue-restricted expression of the cardiac alpha-myosin heavy chain gene is controlled by a downstream repressor element containing a palindrome of two ets-binding sites.Molecular and cellular biology · 1998Article
- Chromatin remodelling of the cardiac beta-myosin heavy chain gene.The Biochemical journal · 1998Article
- Multiple muscle-specific regulatory elements are associated with a DNase I hypersensitive site of the cardiac beta-myosin heavy-chain gene.The Biochemical journal · 1997Article
- Position independent expression and developmental regulation is directed by the beta myosin heavy chain gene's 5' upstream region in transgenic mice.Nucleic acids research · 1995Article
- Segregation of cardiac and skeletal muscle-specific regulatory elements of the beta-myosin heavy chain gene.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Both a ubiquitous factor mTEF-1 and a distinct muscle-specific factor bind to the M-CAT motif of the myosin heavy chain beta gene.Nucleic acids research · 1993Article
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4 authors at 3 institutions in 1 country.
Funding
Abstract
The 5' flanking region of the rabbit myosin heavy chain (HC) beta gene extending 295 bp upstream from the cap site provides muscle-specific transcriptional activity. In this study, we have identified and functionally characterized cis-acting elements that regulate the muscle-specific expression within this region. By using linker-scanner (LS) mutants between -295 bp and a putative TATA box, we found five distinct positive cis-acting sequences necessary for transcription: element A, the sequences between -276 and -263, which contains a putative M-CAT motif in an inverted orientation; B, the sequences between -207 and -180; C, the sequences between -136 and -127; D, the sequences between -91 and -80; and E, a TATA consensus sequence at -28. The fragment containing both A and B elements dramatically enhanced the expression of the chloramphenicol acetyltransferase (CAT) gene driven by a heterologous promoter in differentiated muscle cells, whereas fragments containing either A or B elements alone had little or no effect in either muscle or nonmuscle cells. Therefore, these two elements appear to act cooperatively in determining a high level of muscle- and stage-specific expression. Unlike the typical enhancer element, this region functions in an orientation-dependent manner. In contrast, the fragment containing C and D elements activates the heterologous promoter in both muscle and nonmuscle cells in an orientation-independent manner.
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