ArticleNucleic acids research1992
Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications.
Article in Nucleic acids research, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 153 papers.
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Who cites it
153 citing papers in PubMed.
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- Preparation and application of Taq DNA polymerase monoclonal antibody guided by structural domain analysis.PloS one · 2026Article
- Detection of Viruses with Oncogenic and Oncomodulatory Potential in Head and Neck Tumors-External Auricle.Biomedicines · 2025Article
- Optogenetics with Atomic Precision─A Comprehensive Review of Optical Control of Protein Function through Genetic Code Expansion.Chemical reviews · 2025Review
- Concordance of an in-house 2-steps PCR-SSP and nanopore sequencing forFrontiers in genetics · 2025Article
- Simultaneous Detection of Three Subgroups of Avian Leukosis Virus Using the Nanoparticle-Assisted PCR Assay.Viruses · 2023Article
- Poly(ethylene glycol)-Engrafted Graphene Oxide for Gene Delivery and Nucleic Acid Amplification.Materials (Basel, Switzerland) · 2023Review
- Thermally controlled intein splicing of engineered DNA polymerases provides a robust and generalizable solution for accurate and sensitive molecular diagnostics.Nucleic acids research · 2023Article
- Multiplex PCR assay for the simultaneous identification of race specific and non-specific leaf resistance genes in wheat (Triticum aestivum L.).Journal of applied genetics · 2023Article
- Inhibition of the PCR by genomic DNA.PloS one · 2023Article
- A Simplified Multiplex PCR Assay for Simultaneous Detection of Six Viruses Infecting Diverse Chilli Species in India and Its Application in Field Diagnosis.Pathogens (Basel, Switzerland) · 2022Article
- Developing a multiplex PCR-based assay kit for bloodstream infection by analyzing genomic big data.Journal of clinical laboratory analysis · 2022Article
- Development of Cooperative Primer-Based Real-Time PCR Assays for the Detection of Plasmodium malariae and Plasmodium ovale.The Journal of molecular diagnostics : JMD · 2021Article
- Article
- A blueprint for academic laboratories to produce SARS-CoV-2 quantitative RT-PCR test kits.The Journal of biological chemistry · 2020Article
- A blueprint for academic labs to produce SARS-CoV-2 RT-qPCR test kits.medRxiv : the preprint server for health sciences · 2020Article
- One-Step Nucleic Acid Purification and Noise-Resistant Polymerase Chain Reaction by Electrokinetic Concentration for Ultralow-Abundance Nucleic Acid Detection.Angewandte Chemie (International ed. in English) · 2020Article
- Eliminating primer dimers and improving SNP detection using self-avoiding molecular recognition systems.Biology methods & protocols · 2020Article
- An Alternative Hot Start PCR Method Using a Nuclease-Deficient ExoIII from Escherichia coli.Molecular biotechnology · 2019Article
93 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A Hot Start Polymerase Chain Reaction (PCR) entails the withholding of at least one reagent from the reaction mixture until the reaction tube temperature has reached 60-80 degrees C. Hot Start amplification with an AmpliWax vapor barrier uses a layer of solid wax to separate the retained reagent(s) and the test sample from the bulk of the reagents until the first heating step of automated thermal cycling melts the wax and convectively mixes the two aqueous layers. Wax-mediated Hot Start PCR greatly increases the specificity, yield, and precision of amplifying low copy numbers of three HIV targets. In the presence of 1 microgram of human placental DNA (1.6 x 10(5) diploid genomes) the specificity improvement entails considerable to complete reduction in the amplification of mis-primed sequences and putative primer oligomers. When mis-priming is negligible, the procedural improvement still suppresses putative primer oligomerization. Hot Start PCR with an AmpliWax vapor barrier permits routine amplification of a single target molecule with detection by ethidium stained gel electrophoresis; nonisotopically visualized probing suffices for confirmation. The improved amplification performance is evident for target copy numbers below approximately 10(3).
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