ArticleThe American journal of pathology2005
Macrophage conditioned medium induces the expression of C-reactive protein in human aortic endothelial cells: potential for paracrine/autocrine effects.
Article in The American journal of pathology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
56 citing papers in PubMed, 155 citations in OpenAlex.
- C-reactive protein is expressed and secreted by peripheral blood mononuclear cells.Clinical and experimental immunology · 2006Trial
- Biomarkers of Cardiovascular Stress: A Historical Review.Journal of cardiovascular development and disease · 2026Review
- CRP (C-Reactive Protein) Revisited: An Old Yet New Biomarker of Acute and Chronic Inflammation.Cells · 2026Review
- Six Weeks of Boxing Training Lowers Blood Pressure and Improves Vascular Function in Young Men and Women with Elevated Blood Pressure or Stage 1 Hypertension.Sports (Basel, Switzerland) · 2026Article
- Conditioned Medium from Estradiol-Primed Macrophages Mitigates Adjuvant-Induced Arthritis in Rats.Advanced pharmaceutical bulletin · 2025Article
- Neutrophil Percentage-to-Albumin Ratio as a Predictor of Collateral Circulation in Chronic Total Occlusion.Anatolian journal of cardiology · 2025Article
- The Relationship Between Neutrophil/Lymphocyte Ratio Before Coronary Angiography and Coronary Collateral Development.Cureus · 2025Article
- C-Reactive Protein, Uric Acid, and Coronary Artery Ectasia in Patients with Coronary Artery Disease.Medical science monitor : international medical journal of experimental and clinical research · 2025Article
- Retro walking treadmill training reduces C-reactive protein levels in overweight and obese young adults: A randomized comparative study.Health science reports · 2023Article
- Monomeric C-reactive protein as a biomarker for major depressive disorder.Frontiers in psychiatry · 2023Review
- A biofunctional review of C-reactive protein (CRP) as a mediator of inflammatory and immune responses: differentiating pentameric and modified CRP isoform effects.Frontiers in immunology · 2023Review
- Regulation of Conformational Changes in C-reactive Protein Alters its Bioactivity.Cell biochemistry and biophysics · 2022Review
- Emerging Protein Biomarkers for the Diagnosis or Prediction of Gestational Diabetes-A Scoping Review.Journal of clinical medicine · 2021Article
- Unactivated leukocyte expression of C-reactive protein is minimal and not dependent on rs1205 genotype.Scientific reports · 2021Article
- The secretome mouse provides a genetic platform to delineate tissue-specific in vivo secretion.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- The Effect of Race and Shear Stress on CRP-Induced Responses in Endothelial Cells.Mediators of inflammation · 2021Article
- Ligature induced periodontitis causes atherosclerosis in rat descending aorta: an experimental study.Medicine and pharmacy reports · 2021Article
- Monocytes enhance the inflammatory response to TLR2 stimulation in aortic valve interstitial cells through paracrine up-regulation of TLR2 level.International journal of biological sciences · 2020Article
- C-reactive protein gene rs1205 polymorphism is associated with low-grade chronic inflammation in postmenopausal women.Women's midlife health · 2020Article
- Ligature induced periodontitis causes atherosclerosis in rat descending aorta: an experimental study.Medicine and pharmacy reports · 2019Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
C-reactive protein (CRP) is a risk marker for cardiovascular events in apparently healthy persons. Cogent data show that, aside from the liver, CRP is produced in atherosclerotic lesions, kidney, neurons, and alveolar macrophages. Because several proatherogenic effects of CRP have been documented in endothelial cells, we examined human aortic endothelial cells (HAEC) for CRP production. We detected the presence of CRP mRNA by RT-PCR and in situ hybridization, intracellular protein by Western blot and secreted protein by ELISA. Coincubation with the cytokines interleukin (IL)-1, IL-6, and tumor necrosis factor alone and in combination showed that the most potent agonist for CRP production from HAEC is the combination of IL-1 and IL-6 (P < 0.05). To mimic the in vivo situation, we examined whether vascular smooth muscle cell (VSMC) and/or macrophage conditioned media (MCM) could augment CRP production by HAEC. While VSMC-conditioned media had no effect, incubation with MCM resulted in a significant twofold increase in the synthesis of both intracellular and secreted CRP (P < 0.05). The effect of MCM could be reversed by inhibiting both IL-1 and IL-6. Thus, stimulated synthesis and secretion of CRP by cells in the atherosclerotic lesion by paracrine/autocrine loops could result in local concentrations of CRP far in excess of plasma concentrations and could contribute to proinflammatory, proatherogenic effects.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.