ArticleThe American journal of pathology2005
Chromatin organization measured by AluI restriction enzyme changes with malignancy and is regulated by the extracellular matrix and the cytoskeleton.
Article in The American journal of pathology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 48 citations in OpenAlex.
- On the Complexity of Mechanisms and Consequences of Chromothripsis: An Update.Frontiers in genetics · 2019Review
- Herpes Simplex Virus 1 Infection Promotes the Growth of a Subpopulation of Tumor Cells in Three-Dimensional Uveal Melanoma Cultures.Journal of virology · 2018Article
- Orientation and repositioning of chromosomes correlate with cell geometry-dependent gene expression.Molecular biology of the cell · 2017Article
- Article
- Stem cell marker CD271 is expressed by vasculogenic mimicry-forming uveal melanoma cells in three-dimensional cultures.Molecular vision · 2012Article
- Focal adhesion-chromatin linkage controls tumor cell resistance to radio- and chemotherapy.Chemotherapy research and practice · 2012Article
- Micromechanics of human mitotic chromosomes.Physical biology · 2011Article
- Identification of virus resistant tumor cell subpopulations in three-dimensional uveal melanoma cultures.Cancer gene therapy · 2010Article
- Gene expression in the third dimension: the ECM-nucleus connection.Journal of mammary gland biology and neoplasia · 2010Review
- Hyperactivated NF-{kappa}B and AP-1 transcription factors promote highly accessible chromatin and constitutive transcription across the interleukin-6 gene promoter in metastatic breast cancer cells.Molecular and cellular biology · 2009Article
- Contributions of extracellular matrix signaling and tissue architecture to nuclear mechanisms and spatial organization of gene expression control.Biochimica et biophysica acta · 2009Review
- Authenticating cell lines in ophthalmic research laboratories.Investigative ophthalmology & visual science · 2008Review
- Micromechanical studies of mitotic chromosomes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2008Review
- Modeling the behavior of uveal melanoma in the liver.Investigative ophthalmology & visual science · 2007Article
- The morphologies of breast cancer cell lines in three-dimensional assays correlate with their profiles of gene expression.Molecular oncology · 2007Article
- Epigenetic reversion of breast carcinoma phenotype is accompanied by changes in DNA sequestration as measured by AluI restriction enzyme.The American journal of pathology · 2007Article
- Role of tumor invasiveness, the extracellular matrix, and chromatin sequestration in the susceptibility of uveal melanoma to herpes simplex virus type 1.Experimental eye research · 2007Article
- Different chromatin organization in benign and malignant cells revealed by unequal nuclease sensitivity between tumor and normal cell genomes.The American journal of pathology · 2007Article
- Extracellular matrix, nuclear and chromatin structure, and gene expression in normal tissues and malignant tumors: a work in progress.Advances in cancer research · 2007Review
- Tumor cell plasticity in uveal melanoma: microenvironment directed dampening of the invasive and metastatic genotype and phenotype accompanies the generation of vasculogenic mimicry patterns.The American journal of pathology · 2006Article
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11 authors at 2 institutions in 1 country.
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Abstract
Given that expression of many genes changes when cells become malignant or are placed in different microenvironments, we asked whether these changes were accompanied by global reorganization of chromatin. We reasoned that sequestration or exposure of chromatin-sensitive sites to restriction enzymes could be used to detect this reorganization. We found that AluI-sensitive sites of nonmalignant cells were relatively more exposed compared to their malignant counterparts in cultured cells and human tumor samples. Changes in exposure and sequestration of AluI-sensitive sites in normal fibroblasts versus fibrosarcoma or those transfected with oncogenes, nonmalignant breast cells versus carcinomas and poorly metastatic versus highly invasive melanoma were shown to be independent of the cell cycle and may be influenced by proteins rich in disulfide bonds. Remarkably, regardless of degree of malignancy, AluI-sensitive sites became profoundly sequestered when cells were incubated with laminin, Matrigel, or a circular RGD peptide (RGD-C), but became exposed when cells were placed on collagen I or in serum-containing medium. Disruption of the actin cytoskeleton led to exposure, whereas disruption of microtubules or intermediate filaments exerted a sequestering effect. Thus, AluI-sensitive sites are more sequestered with increasing malignant behavior, but the sequestration and exposure of these sites is exquisitely sensitive to information conferred to the cell by molecules and biomechanical forces that regulate cellular and tissue architecture.
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