ArticleAntimicrobial agents and chemotherapy2005
Expression of ccaR, encoding the positive activator of cephamycin C and clavulanic acid production in Streptomyces clavuligerus, is dependent on bldG.
Article in Antimicrobial agents and chemotherapy, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 60 citations in OpenAlex.
- Bacterial biosynthetic superclusters: emerging drivers of synergistic natural products against antimicrobial resistance.Essays in biochemistry · 2026Review
- Beyond Clavulanic Acid biosynthesis: Exploring the broad regulatory impact of BldD in Streptomyces clavuligerus ATCC 27064.PloS one · 2026Article
- Global regulator AdpA directly binds to tunicamycin gene cluster and negatively regulates tunicamycin biosynthesis in Streptomyces clavuligerus.World journal of microbiology & biotechnology · 2024Article
- An integrative-omics analysis of an industrial clavulanic acid-overproducing Streptomyces clavuligerus.Applied microbiology and biotechnology · 2022Article
- Streptomyces clavuligerus: The Omics Era.Journal of industrial microbiology & biotechnology · 2021Article
- Identification of Novel Rotihibin Analogues inMicrobiology spectrum · 2021Article
- Clavulanic Acid Production byAntibiotics (Basel, Switzerland) · 2021Review
- Homologous expression of lysA encoding diaminopimelic acid (DAP) decarboxylase reveals increased antibiotic production in Streptomyces clavuligerus.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Article
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- Comparative Transcriptome Analysis ofAntibiotics (Basel, Switzerland) · 2019Article
- The CagRS Two-Component System Regulates Clavulanic Acid Metabolism via Multiple Pathways inFrontiers in microbiology · 2019Article
- Contribution of the β-glucosidase BglC to the onset of the pathogenic lifestyle of Streptomyces scabies.Molecular plant pathology · 2018Article
- Proteomics analysis of global regulatory cascades involved in clavulanic acid production and morphological development in Streptomyces clavuligerus.Journal of industrial microbiology & biotechnology · 2016Article
- Regulation of antimycin biosynthesis by the orphan ECF RNA polymerase sigma factor σ (AntA.).PeerJ · 2014Article
- Engineered Streptomyces avermitilis host for heterologous expression of biosynthetic gene cluster for secondary metabolites.ACS synthetic biology · 2013Article
- Biosynthesis of clavam metabolites.Journal of industrial microbiology & biotechnology · 2012Review
- Molecular Analysis of the Clavulanic Acid Regulatory Gene Isolated from an Iranian Strain of Streptomyces Clavuligerus , PTCC 1709.Cell journal · 2011Article
- Genome-minimized Streptomyces host for the heterologous expression of secondary metabolism.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- BldG and SCO3548 interact antagonistically to control key developmental processes in Streptomyces coelicolor.Journal of bacteriology · 2009Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Streptomyces coelicolor, bldG encodes a putative anti-anti-sigma factor that regulates both aerial hypha formation and antibiotic production, and a downstream transcriptionally linked open reading frame (orf3) encodes a putative anti-sigma factor protein. A cloned DNA fragment from Streptomyces clavuligerus contained an open reading frame that encoded a protein showing 92% identity to the S. coelicolor BldG protein and 91% identity to the BldG ortholog in Streptomyces avermitilis. Sequencing of the region downstream of bldG in S. clavuligerus revealed the presence of an open reading frame encoding a protein showing 72 and 69% identity to the ORF3 proteins in S. coelicolor and S. avermitilis, respectively. Northern analysis indicated that, as in S. coelicolor, the S. clavuligerus bldG gene is expressed as both a monocistronic and a polycistronic transcript, the latter including the downstream orf3 gene. High-resolution S1 nuclease mapping of S. clavuligerus bldG transcripts revealed the presence of three bldG-specific promoters, and analysis of expression of a bldGp-egfp reporter indicated that the bldG promoter is active at various stages of development and in both substrate and aerial hyphae. A bldG null mutant was defective in both morphological differentiation and in the production of secondary metabolites, such as cephamycin C, clavulanic acid, and the 5S clavams. This inability to produce cephamycin C and clavulanic acid was due to the absence of the CcaR transcriptional regulator, which controls the expression of biosynthetic genes for both secondary metabolites as well as the expression of a second regulator of clavulanic acid biosynthesis, ClaR. This makes bldG the first regulatory protein identified in S. clavuligerus that functions upstream of CcaR and ClaR in a regulatory cascade to control secondary metabolite production.
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