ArticlePlant physiology2005
GmN70 and LjN70. Anion transporters of the symbiosome membrane of nodules with a transport preference for nitrate.
Article in Plant physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 49 citations in OpenAlex.
- Review
- Review
- Membrane Proteins in Plant Salinity Stress Perception, Sensing, and Response.The Journal of membrane biology · 2023Review
- A roadmap of plant membrane transporters in arbuscular mycorrhizal and legume-rhizobium symbioses.Plant physiology · 2021Review
- PHO1 family members transport phosphate from infected nodule cells to bacteroids in Medicago truncatula.Plant physiology · 2021Article
- A GMC Oxidoreductase GmcA Is Required for Symbiotic Nitrogen Fixation inFrontiers in microbiology · 2020Article
- Transcriptome analysis of soybean (Glycine max) root genes differentially expressed in rhizobial, arbuscular mycorrhizal, and dual symbiosis.Journal of plant research · 2019Article
- An Iron-Activated Citrate Transporter, MtMATE67, Is Required for Symbiotic Nitrogen Fixation.Plant physiology · 2018Article
- The plasma membrane proteome of Medicago truncatula roots as modified by arbuscular mycorrhizal symbiosis.Mycorrhiza · 2018Article
- The Nitrate Transporter Family Protein LjNPF8.6 Controls the N-Fixing Nodule Activity.Plant physiology · 2017Article
- The Symbiosome: Legume and Rhizobia Co-evolution toward a Nitrogen-Fixing Organelle?Frontiers in plant science · 2017Review
- Transport processes of the legume symbiosome membrane.Frontiers in plant science · 2014Article
- Article
- A role for nonsense-mediated mRNA decay in plants: pathogen responses are induced in Arabidopsis thaliana NMD mutants.PloS one · 2012Article
- HvNax3--a locus controlling shoot sodium exclusion derived from wild barley (Hordeum vulgare ssp. spontaneum).Functional & integrative genomics · 2010Article
- Channel-like characteristics of the low-affinity barley phosphate transporter PHT1;6 when expressed in Xenopus oocytes.Plant physiology · 2010Article
- Genomic inventory and transcriptional analysis of Medicago truncatula transporters.Plant physiology · 2010Article
- Heterelogous expression of plant genes.International journal of plant genomics · 2009Article
- NIP6;1 is a boric acid channel for preferential transport of boron to growing shoot tissues in Arabidopsis.The Plant cell · 2008Article
- Nutrient sharing between symbionts.Plant physiology · 2007Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A cDNA was isolated from soybean (Glycine max) nodules that encodes a putative transporter (GmN70) of the major facilitator superfamily. GmN70 is expressed predominantly in mature nitrogen-fixing root nodules. By western-blot and immunocytochemical analyses, GmN70 was localized to the symbiosome membrane of infected root nodule cells, suggesting a transport role in symbiosis. To investigate its transport function, cRNA encoding GmN70 was expressed in Xenopus laevis oocytes, and two-electrode voltage clamp analysis was performed. Ooctyes expressing GmN70 showed outward currents that are carried by anions with a selectivity of nitrate > nitrite > > chloride. These currents showed little sensitivity to pH or the nature of the counter cation in the oocyte bath solution. One-half maximal currents were induced by nitrate concentrations between 1 to 3 mm. No apparent transport of organic anions was observed. Voltage clamp records of an ortholog of GmN70 from Lotus japonicus (LjN70; K. Szczyglowski, P. Kapranov, D. Hamburger, F.J. de Bruijn [1998] Plant Mol Biol 37: 651-661) also showed anion currents with a similar selectivity profile. Overall, these findings suggest that GmN70 and LjN70 are inorganic anion transporters of the symbiosome membrane with enhanced preference for nitrate. These transport activities may aid in regulation of ion and membrane potential homeostasis, possibly in response to external nitrate concentrations that are known to regulate the symbiosis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.