ArticlePlant physiology2005
Ectopic expression of an amino acid transporter (VfAAP1) in seeds of Vicia narbonensis and pea increases storage proteins.
Article in Plant physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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55 citing papers in PubMed, 192 citations in OpenAlex.
- Rational gene stacking for seed-specific carbon partitioning to simultaneously enhance lipid and protein contents in Arabidopsis.Journal of experimental botany · 2026Article
- High-throughput screening for biostimulant discovery: opportunities to enhance nitrogen use efficiency.Frontiers in plant science · 2026Review
- Seed the Difference: QTL Mapping Reveals Several Major Loci for Seed Size inPlants (Basel, Switzerland) · 2025Article
- Target of rapamycin signaling in pea embryos is dependent on glutamine but detached from seed storage protein biosynthesis.The New phytologist · 2025Article
- Genetic Improvement and Functional Characterization of AAP1 Gene for Enhancing Nitrogen Use Efficiency in Maize.Plants (Basel, Switzerland) · 2025Article
- QTL mapping and multi-omics identify candidate genes for protein and oil in soybean.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Genetic variation in storage protein and starch biosynthesis genes reveals key associations with seed composition in pea (Frontiers in plant science · 2025Article
- Knocking OutInternational journal of molecular sciences · 2023Article
- Genome-Wide Analysis of the Amino Acid Permeases Gene Family in Wheat andInternational journal of molecular sciences · 2023Article
- Selection pressure on the rhizosphere microbiome can alter nitrogen use efficiency and seed yield in Brassica rapa.Communications biology · 2022Article
- Transcription Factor DOF4.1 Regulates Seed Longevity in ArabidopsisFrontiers in plant science · 2022Article
- Review
- Temporal Control of Seed Development in Dicots: Molecular Bases, Ecological Impact and Possible Evolutionary Ramifications.International journal of molecular sciences · 2021Review
- The tea plant CsLHT1 and CsLHT6 transporters take up amino acids, as a nitrogen source, from the soil of organic tea plantations.Horticulture research · 2021Article
- Article
- Wheat amino acid transporters highly expressed in grain cells regulate amino acid accumulation in grain.PloS one · 2021Article
- Genomic regions associated with important seed quality traits in food-grade soybeans.BMC plant biology · 2020Article
- Overexpression of GmAAP6a enhances tolerance to low nitrogen and improves seed nitrogen status by optimizing amino acid partitioning in soybean.Plant biotechnology journal · 2020Article
- Amino Acid Transporters in Plant Cells: A Brief Review.Plants (Basel, Switzerland) · 2020Review
- Evolutionary significance of amino acid permease transporters in 17 plants from Chlorophyta to Angiospermae.BMC genomics · 2020Article
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10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Storage protein synthesis is dependent on available nitrogen in the seed, which may be controlled by amino acid import via specific transporters. To analyze their rate-limiting role for seed protein synthesis, a Vicia faba amino acid permease, VfAAP1, has been ectopically expressed in pea (Pisum sativum) and Vicia narbonensis seeds under the control of the legumin B4 promoter. In mature seeds, starch is unchanged but total nitrogen is 10% to 25% higher, which affects mainly globulin, vicilin, and legumin, rather than albumin synthesis. Transgenic seeds in vitro take up more [14C]-glutamine, indicating increased sink strength for amino acids. In addition, more [14C] is partitioned into proteins. Levels of total free amino acids in growing seeds are unchanged but with a shift toward higher relative abundance of asparagine, aspartate, glutamine, and glutamate. Hexoses are decreased, whereas metabolites of glycolysis and the tricarboxylic acid cycle are unchanged or slightly lower. Phosphoenolpyruvate carboxylase activity and the phosphoenolpyruvate carboxylase-to-pyruvate kinase ratios are higher in seeds of one and three lines, indicating increased anaplerotic fluxes. Increases of individual seed size by 20% to 30% and of vegetative biomass indicate growth responses probably due to improved nitrogen status. However, seed yield per plant was not altered. Root application of [15N] ammonia results in significantly higher label in transgenic seeds, as well as in stems and pods, and indicates stimulation of nitrogen root uptake. In summary, VfAAP1 expression increases seed sink strength for nitrogen, improves plant nitrogen status, and leads to higher seed protein. We conclude that seed protein synthesis is nitrogen limited and that seed uptake activity for nitrogen is rate limiting for storage protein synthesis.
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