ArticleMarine biotechnology (New York, N.Y.)
PCR-based approach for sequencing mitochondrial genomes of decapod crustaceans, with a practical example from kuruma prawn (Marsupenaeus japonicus).
Article in Marine biotechnology (New York, N.Y.). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 57 citations in OpenAlex.
- The complete mitochondrial genome and phylogenetic analysis of the Jawala paste shrimp,Mitochondrial DNA. Part B, Resources · 2026Article
- The complete mitochondrial genome ofMitochondrial DNA. Part B, Resources · 2026Article
- Article
- Genome and transcriptome assemblies of the kuruma shrimp, Marsupenaeus japonicus.G3 (Bethesda, Md.) · 2021Article
- Article
- The complete mitochondrial genome of the cryptic species (Form II) in kuruma shrimpMitochondrial DNA. Part B, Resources · 2018Article
- Development of a Method to Extract and Amplify the Complete Mitogenome of Some Sparidae Species.Italian journal of food safety · 2017Article
- Article
- Evolution and phylogeny of the mud shrimps (Crustacea: Decapoda) revealed from complete mitochondrial genomes.BMC genomics · 2012Article
- The mitochondrial genomes of Euphausia pacifica and Thysanoessa raschii sequenced using 454 next-generation sequencing, with a phylogenetic analysis of their position in the Malacostracan family tree.Molecular biology reports · 2012Article
- The mitochondrial genome of Euphausia superba (Prydz Bay) (Crustacea: Malacostraca: Euphausiacea) reveals a novel gene arrangement and potential molecular markers.Molecular biology reports · 2010Article
- Monophyletic origin of domestic bactrian camel (Camelus bactrianus) and its evolutionary relationship with the extant wild camel (Camelus bactrianus ferus).Animal genetics · 2009Article
- Article
- Systematic sequencing of mRNA from the Antarctic krill (Euphausia superba) and first tissue specific transcriptional signature.BMC genomics · 2008Article
- The complete mitochondrial genome of Pseudocellus pearsei (Chelicerata: Ricinulei) and a comparison of mitochondrial gene rearrangements in Arachnida.BMC genomics · 2007Article
- A complete mitochondrial genome sequence of the wild two-humped camel (Camelus bactrianus ferus): an evolutionary history of camelidae.BMC genomics · 2007Article
- The complete mitochondrial genome of the common sea slater, Ligia oceanica (Crustacea, Isopoda) bears a novel gene order and unusual control region features.BMC genomics · 2006Article
- A male reproduction-related Kazal-type peptidase inhibitor gene in the prawn, Macrobrachium rosenbergii: molecular characterization and expression patterns.Marine biotechnology (New York, N.Y.)Article
- Analysis of the secondary structure of ITS1 in Pectinidae: implications for phylogenetic reconstruction and structural evolution.Marine biotechnology (New York, N.Y.)Article
- Organization of the mitochondrial genome of mantis shrimp Pseudosquilla ciliata (Crustacea: Stomatopoda).Marine biotechnology (New York, N.Y.)Article
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An approach for sequencing the entire mitochondrial genomes (mitogenomes) of decapod crustaceans using 79 newly designed and 7 published polymerase chain reaction (PCR) primers is described. The approach comprises the following steps: (1) the entire mitogenome is amplified in 2 or 3 long PCRs; (2) the 86 primers are used in different combinations to amplify contiguous, overlapping short segments of the entire mitogenome with the diluted long PCR products as templates; (3) direct cycle sequencing is conducted using the short PCR products. This strategy allows a more rapid determination of decapod mitogenomic sequences than a traditional method using cloned mitochondrial DNA and primer walking strategy. As a practical example, the mitogenomic sequence for a kuruma prawn Marsupenaeus japonicus (Crustacea: Decapoda), was determined using the PCR-based approach.
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