ArticleInvestigative ophthalmology & visual science2005
Subcellular redistribution of components of the ubiquitin-proteasome pathway during lens differentiation and maturation.
Article in Investigative ophthalmology & visual science, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 32 citations in OpenAlex.
- Functional expression and localisation of HOPS/TMUB1 in mouse lens.Bioscience reports · 2021Article
- Uncovering mechanisms of nuclear degradation in keratinocytes: A paradigm for nuclear degradation in other tissues.Nucleus (Austin, Tex.) · 2018Review
- Identification and Ultrastructural Characterization of a Novel Nuclear Degradation Complex in Differentiating Lens Fiber Cells.PloS one · 2016Article
- Connexin Controls Cell-Cycle Exit and Cell Differentiation by Directly Promoting Cytosolic Localization and Degradation of E3 Ligase Skp2.Developmental cell · 2015Article
- Role of αA-crystallin-derived αA66-80 peptide in guinea pig lens crystallin aggregation and insolubilization.Experimental eye research · 2015Article
- Newborn mouse lens proteome and its alteration by lysine 6 mutant ubiquitin.Journal of proteome research · 2014Article
- Autophagy and mitophagy participate in ocular lens organelle degradation.Experimental eye research · 2013Article
- Oligomerization with wt αA- and αB-crystallins reduces proteasome-mediated degradation of C-terminally truncated αA-crystallin.Investigative ophthalmology & visual science · 2012Article
- Ubiquitin-proteasome system components are upregulated during intestinal regeneration.Genesis (New York, N.Y. : 2000) · 2012Article
- Survivin expression is associated with lens epithelial cell proliferation and fiber cell differentiation.Molecular vision · 2012Article
- Lens fibre cell differentiation and organelle loss: many paths lead to clarity.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2011Review
- Nuclear import of an intact preassembled proteasome particle.Molecular biology of the cell · 2011Article
- Ubiquitin proteasome pathway-mediated degradation of proteins: effects due to site-specific substrate deamidation.Investigative ophthalmology & visual science · 2010Article
- Increased O-GlcNAc causes disrupted lens fiber cell differentiation and cataracts.Biochemical and biophysical research communications · 2009Article
- On the mechanism of organelle degradation in the vertebrate lens.Experimental eye research · 2009Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
purposeTo determine the subcellular distribution of components of the ubiquitin-proteasome pathway (UPP) in lens epithelium and differentiating fibers and to evaluate potential roles of the UPP in eliminating nuclei and other organelles during maturation of lens fibers.
methodsAdult bovine lens cryosections were stained for immunofluorescence and analyzed by confocal microscopy. The specificities of the antibodies used in this study were determined by Western blot. results Cryosections of bovine lenses show that E1 and Ubc1 were present in both the cytoplasm and the nucleus in epithelial cells, whereas Ubc3 and ubiquitin conjugates were mostly confined to the nucleus, and Ubc4/5 was preferentially localized in clusters in the vicinity of the nuclear membrane. The 19S and 20S proteasome complexes were preferentially localized in the cytoplasm. When the epithelial cells differentiated into fiber cells at the transition zone, all components of the UPP were primarily present in the nucleus, with the exception of Ubc4/5, which was associated with the nuclear membrane. conclusions The results show that during lens fiber differentiation and maturation, components of the UPP are redistributed at subcellular levels. Subcellular localization of an enzyme indicates where the reaction takes place. The primary nuclear localization of the UPP components in the differentiating fibers supports the hypothesis that the UPP may play a role in elimination of nuclei and other organelles during differentiation and maturation of lens fibers.
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