ArticleJournal of clinical pathology2005
Differences in growth characteristics and elementary body associated cytotoxicity between Chlamydia trachomatis oculogenital serovars D and H and Chlamydia muridarum.
Article in Journal of clinical pathology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 43 citations in OpenAlex.
- Article
- Recognition ofInfection and immunity · 2024Article
- Direct assessment of possible mutations in the 23S rRNA gene encoding macrolide resistance in Chlamydia trachomatis.PloS one · 2022Article
- Host-Pathogen Interactions ofPathogens (Basel, Switzerland) · 2021Article
- Dual RNA-seq analysis of in vitro infection multiplicity and RNA depletion methods in Chlamydia-infected epithelial cells.Scientific reports · 2021Article
- Article
- Growth kinetics of Chlamydia trachomatis in primary human Sertoli cells.Scientific reports · 2019Article
- Article
- Effect of cold water-induced stress on immune response, pathology and fertility in mice during Chlamydia muridarum genital infection.Pathogens and disease · 2017Article
- Comparison of Murine Cervicovaginal Infection by Chlamydial Strains: Identification of Extrusions ShedFrontiers in cellular and infection microbiology · 2017Article
- Article
- Recombinant Human Peptidoglycan Recognition Proteins Reveal Antichlamydial Activity.Infection and immunity · 2016Article
- TLR2, TLR4 and TLR9 genotypes and haplotypes in the susceptibility to and clinical course of Chlamydia trachomatis infections in Dutch women.Pathogens and disease · 2016Article
- A Multi-Component Prime-Boost Vaccination Regimen with a Consensus MOMP Antigen Enhances Chlamydia trachomatis Clearance.Frontiers in immunology · 2016Article
- Genital Chlamydia trachomatis: understanding the roles of innate and adaptive immunity in vaccine research.Clinical microbiology reviews · 2014Review
- Serovar D and E of serogroup B induce highest serological responses in urogenital Chlamydia trachomatis infections.BMC infectious diseases · 2014Article
- Animal models for studying female genital tract infection with Chlamydia trachomatis.Infection and immunity · 2013Review
- Chlamydia trachomatis vaccine research through the years.Infectious diseases in obstetrics and gynecology · 2011Review
- Discovering and differentiating new and emerging clonal populations of Chlamydia trachomatis with a novel shotgun cell culture harvest assay.Emerging infectious diseases · 2008Article
- Efficacy of an immune modulator in experimental Chlamydia trachomatis infection of the female genital tract.Infectious diseases in obstetrics and gynecology · 2006Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimIn vitro growth and elementary body (EB) associated cytotoxicity of two Chlamydia trachomatis strains belonging to serovars D and H and C muridarum were compared to identify difference(s) that correlate with virulence variations between these strains in the mouse model of human female genital tract infection, and phenotypic characteristics that could explain human epidemiological data on serovar prevalence and levels of shedding during serovar D and H infection.
methodsReplication cycle kinetics, inclusion characteristics, and EB associated cytotoxicity were assessed in McCoy cell monolayers using culture, light microscopy, and lactate dehydrogenase release.
resultsOver 72 hours, more rapid production and release of inclusion forming units (ifu) allowed C muridarum to initiate two replication rounds, resulting in 4-8 times more ifu/input unit of infection than with serovars D and H. Although C muridarum EBs were significantly more cytotoxic to McCoy cell monolayers than serovar D at moderate and high multiplicity of infection ratios (MOI), serovar H EBs were significantly more cytotoxic than C muridarum, even at the lowest MOI tested.
conclusionsThese phenotypic differences are consistent with the more invasive course and severe pathological outcome of infection in mice infected with C muridarum, providing an objective basis for questioning the appropriateness of C muridarum as a surrogate for the human biovar of C trachomatis in the murine model of female genital tract infection. The differences seen between the human strains could help explain human epidemiological data relating to differences in prevalence and level of shedding that occurs during infection with oculogenital serovars D and H.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.