ArticleVirology journal2005
Typing of human rotaviruses: nucleotide mismatches between the VP7 gene and primer are associated with genotyping failure.
Article in Virology journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Molecular genotyping and quantitation assay for rotavirus surveillance.Journal of virological methods · 2015Article
- Inaccurate identification of rotavirus genotype G9 as genotype G3 strains due to primer mismatch.Virology journal · 2012Article
- In silico study of rotavirus VP7 surface accessible conserved regions for antiviral drug/vaccine design.PloS one · 2012Article
- Rotavirus infection: a perspective on epidemiology, genomic diversity and vaccine strategies.Indian journal of virology : an official organ of Indian Virological Society · 2011Article
- Article
- Rapid changes in rotaviral genotypes in Ecuador.Journal of medical virology · 2009Article
- Genomic characterization of nontypeable rotaviruses and detection of a rare G8 strain in Delhi, India.Journal of clinical microbiology · 2009Article
- Characterization of novel VP7, VP4, and VP6 genotypes of a previously untypeable group A rotavirus.Virology · 2009Article
- Development of a microtiter plate hybridization-based PCR-enzyme-linked immunosorbent assay for identification of clinically relevant human group A rotavirus G and P genotypes.Journal of clinical microbiology · 2008Article
- Development and validation of DNA microarray for genotyping group A rotavirus VP4 (P[4], P[6], P[8], P[9], and P[14]) and VP7 (G1 to G6, G8 to G10, and G12) genes.Journal of clinical microbiology · 2007Article
- Prevalence of G2P[4] and G12P[6] rotavirus, Bangladesh.Emerging infectious diseases · 2007Article
- Nucleotide mismatches between the VP7 gene and the primer are associated with genotyping failure of a specific lineage from G1 rotavirus strains.Virology journal · 2006Article
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRotavirus genotyping is performed by using reverse transcription PCR with type-specific-primers. Because the high rotavirus mutation rate generates an extensive genomic variation, different G-type-specific primer sets are applied in different geographical locations. In Bangladesh, a significant proportion (36.9%) of the rotavirus strains isolated in 2002 could not be G-typed using the routinely used primer set. To investigate the reason why the strains were untypeable, nucleotide sequencing of the VP7 genes was performed.
resultsFour nucleotide substitutions at the G1 primer-binding site of the VP7 gene of Bangladeshi G1 rotaviruses rendered a major proportion of circulating strains untypeable using the routine primer set. Using an alternative primer set, we could identify G1 rotaviruses as the most prevalent genotype (44.8%), followed by G9 (21.7%), G2 (15.0%) and G4 (13.8%).
conclusionBecause of the natural variation in the rotaviral gene sequences, close monitoring of rotavirus genotyping methods is important.
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