ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2005
Phosphorylation-dependent low-frequency depression at phasic synapses of a crayfish motoneuron.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 22 citations in OpenAlex.
- Article
- Frequency-dependent mobilization of heterogeneous pools of synaptic vesicles shapes presynaptic plasticity.eLife · 2017Article
- Article
- The cytoplasmic domain of rat synaptotagmin I enhances synaptic transmission.Cellular and molecular neurobiology · 2014Article
- Calcium, calpain, and calcineurin in low-frequency depression of transmitter release.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Structure/function assessment of synapses at motor nerve terminals.Synapse (New York, N.Y.) · 2011Article
- A novel form of presynaptic plasticity based on the fast reactivation of release sites switched off during low-frequency depression.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Two forms of long-term depression in a polysynaptic pathway in the leech CNS: one NMDA receptor-dependent and the other cannabinoid-dependent.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2009Article
- Facilitating exchange: activity-dependent regulation of presynaptic Na+/Ca2+ exchange.The Journal of physiology · 2008Review
- Okadaic acid-sensitive protein phosphatases constrain phrenic long-term facilitation after sustained hypoxia.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- Depression in the fly.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2006Article
- Cholesterol and synaptic transmitter release at crayfish neuromuscular junctions.The Journal of physiology · 2006Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Extremes in presynaptic differentiation can be studied at the crayfish leg extensor muscle where, on the same muscle fiber, one motoneuron makes "phasic" depressing synapses that have a high probability of neurotransmitter release and another motoneuron makes "tonic," low-probability, facilitating synapses. The large motor axons permit intracellular access to presynaptic sites. We examined the role of phosphorylation during low-frequency depression (LFD) in the relatively little studied phasic synapses. LFD occurs with stimulation at 0.2 Hz and develops with time constants of 4 and 105 min to reach >50% depression of transmitter release in 60 min similar to long-term depression in mammals. LFD is not associated with changes in postsynaptic sensitivity to transmitter and thus is a presynaptic event, although it is not accompanied by changes in the presynaptic action potential. Blockade of protein kinases accelerated the slow phase of LFD, but stimulation of kinases reduced depression. Blockade of protein phosphatases 1A/2A reversed the slow phase. When calcineurin was inhibited, both phases of LFD were abolished, and facilitation occurred instead. Immunostaining showed calcineurin-like immunoreactivity in synaptic terminals. Recovery from LFD occurred in approximately 1 h if stimulation frequency was reduced to 0.0016 Hz. Recovery was blocked by kinase inhibition. This study shows that phosphorylation-dependent mechanisms are involved in LFD and suggests that exocytosis is controlled by conditions that shift the balance between phosphorylated and unphosphorylated substrates. The shift can occur by alteration in the relative activities of protein kinases and phosphatases.
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