ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2005
Mechanisms of transport and exocytosis of dense-core granules containing tissue plasminogen activator in developing hippocampal neurons.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 51 citations in OpenAlex.
- Unraveling the Dynamics of Oxytocin in Hypothalamic Neurons.Traffic (Copenhagen, Denmark) · 2026Article
- Maximal Fusion Capacity and Efficient Replenishment of the Dense Core Vesicle Pool in Hippocampal Neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Modulations of the neuronal trafficking of tissue-type plasminogen activator (tPA) influences glutamate release.Cell death & disease · 2023Article
- Differential axonal trafficking of Neuropeptide Y-, LAMP1-, and RAB7-tagged organelles in vivo.eLife · 2022Article
- Tissue Plasminogen Activator Expression Is Restricted to Subsets of Excitatory Pyramidal Glutamatergic Neurons.Molecular neurobiology · 2016Article
- Stochastic Subcellular Organization of Dense-Core Vesicles Revealed by Point Pattern Analysis.Biophysical journal · 2016Article
- Physiological and pathological roles of tissue plasminogen activator and its inhibitor neuroserpin in the nervous system.Frontiers in cellular neuroscience · 2015Review
- Munc13 controls the location and efficiency of dense-core vesicle release in neurons.The Journal of cell biology · 2012Article
- Hindered submicron mobility and long-term storage of presynaptic dense-core granules revealed by single-particle tracking.Developmental neurobiology · 2012Article
- All three components of the neuronal SNARE complex contribute to secretory vesicle docking.The Journal of cell biology · 2012Article
- Analysis of molecular diffusion by first-passage time variance identifies the size of confinement zones.Biophysical journal · 2011Article
- Dynamics of peptidergic secretory granule transport are regulated by neuronal stimulation.BMC neuroscience · 2010Article
- Localized topological changes of the plasma membrane upon exocytosis visualized by polarized TIRFM.The Journal of cell biology · 2010Article
- BDNF signaling in the formation, maturation and plasticity of glutamatergic and GABAergic synapses.Experimental brain research · 2009Review
- Expression of the dominant-negative tail of myosin Va enhances exocytosis of large dense core vesicles in neurons.Cellular and molecular neurobiology · 2009Article
- Matrix-dependent local retention of secretory vesicle cargo in cortical neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Imaging of evoked dense-core-vesicle exocytosis in hippocampal neurons reveals long latencies and kiss-and-run fusion events.Journal of cell science · 2009Article
- Efficient copackaging and cotransport yields postsynaptic colocalization of neuromodulators associated with synaptic plasticity.Developmental neurobiology · 2008Article
- Signaling for vesicle mobilization and synaptic plasticity.Molecular neurobiology · 2008Review
- Increased motion and travel, rather than stable docking, characterize the last moments before secretory granule fusion.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Dense-core granules (DCGs) are organelles found in specialized secretory cells, including neuroendocrine cells and neurons. Neuronal DCGs facilitate many critical processes, including the transport and secretion of proteins involved in learning, and yet their transport and exocytosis are poorly understood. We have used wide-field and total internal reflection fluorescence microscopy, in conjunction with transport theory, to visualize the transport and exocytosis of DCGs containing a tissue plasminogen activator-green fluorescent protein hybrid in cell bodies, neurites, and growth cones of developing hippocampal neurons and to quantify the roles that diffusion, directed motion, and immobility play in these processes. Our results demonstrate that shorter-ranged transport of DCGs near sites of exocytosis in hippocampal neurons and neuroendocrine cells differs markedly. Specifically, the immobile fraction of DCGs within growth cones and near the plasma membrane of hippocampal neurons is small and relatively unaltered by actin disruption, unlike in neuroendocrine cells. Moreover, transport of DCGs in these domains of hippocampal neurons is unusually heterogeneous, being significantly rapid and directed as well as slow and diffusive. Our results also demonstrate that exocytosis is preceded by substantial movement and heterogeneous transport; this movement may facilitate delivery of DCG cargo in hippocampal neurons, given the relatively low abundance of neuronal DCGs. In addition, the extensive mobility of DCGs in hippocampal neurons argues strongly against the hypothesis that cortical actin is a major barrier to membrane-proximal DCGs in these cells. Instead, our results suggest that extended release of DCG cargo from hippocampal neurons arises from heterogeneity in DCG mobility.
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