ArticleThe British journal of dermatology2005
Corticotropin-releasing hormone triggers differentiation in HaCaT keratinocytes.
Article in The British journal of dermatology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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Who cites it
22 citing papers in PubMed, 46 citations in OpenAlex.
- Neuroendocrine signaling in the skin with a special focus on the epidermal neuropeptides.American journal of physiology. Cell physiology · 2022Review
- Modulation of dermal equivalent of hypothalamus-pituitary-adrenal axis in mastocytosis.Postepy dermatologii i alergologii · 2021Article
- Development of a Peptide Derived from Platelet-Derived Growth Factor (PDGF-BB) into a Potential Drug Candidate for the Treatment of Wounds.Advances in wound care · 2020Article
- Respective optimal calcium concentrations for proliferation on type I collagen fibrils in two keratinocyte line cells, HaCaT and FEPE1L-8.Regenerative therapy · 2018Article
- Association between Stress and the HPA Axis in the Atopic Dermatitis.International journal of molecular sciences · 2017Review
- Stress-related skin disorders.Reviews in endocrine & metabolic disorders · 2016Review
- Current knowledge on psoriasis and autoimmune diseases.Psoriasis (Auckland, N.Z.) · 2016Review
- Article
- Synthesis and Evaluation of Biological Activity of Antimicrobial--Pro-Proliferative Peptide Conjugates.PloS one · 2015Article
- Brain-skin connection: stress, inflammation and skin aging.Inflammation & allergy drug targets · 2014Review
- Key role of CRF in the skin stress response system.Endocrine reviews · 2013Review
- Substance P (SP) induces expression of functional corticotropin-releasing hormone receptor-1 (CRHR-1) in human mast cells.The Journal of investigative dermatology · 2012Article
- Sensing the environment: regulation of local and global homeostasis by the skin's neuroendocrine system.Advances in anatomy, embryology, and cell biology · 2012Review
- The mouse cochlea expresses a local hypothalamic-pituitary-adrenal equivalent signaling system and requires corticotropin-releasing factor receptor 1 to establish normal hair cell innervation and cochlear sensitivity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Neuroendocrinology of the skin: An overview and selective analysis.Dermato-endocrinology · 2011Article
- Emerging role of alternative splicing of CRF1 receptor in CRF signaling.Acta biochimica Polonica · 2010Review
- Detection of intracellular granularity induction in prostate cancer cell lines by small molecules using the HyperCyt high-throughput flow cytometry system.Journal of biomolecular screening · 2009Article
- Cutaneous induction of corticotropin releasing hormone by Propionibacterium acnes extracts.Dermato-endocrinology · 2009Article
- Corticotropin releasing hormone and the skin.Frontiers in bioscience : a journal and virtual library · 2006Review
- Corticotropin releasing factor (CRF) receptor signaling in the central nervous system: new molecular targets.CNS & neurological disorders drug targets · 2006Review
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundCorticotropin-releasing hormone (CRH) is proposed to be involved in the regulation of the proliferative capacity of keratinocytes, based on its significant actions in the skin. These are mediated by CRH-R1alpha and represented by adenylate cyclase activation, Ca2+ influx, inhibition of cell proliferation and modifications in intracellular signal transduction by NF-kappaB.
objectivesTo define CRH action in the cell cycle we investigated its effects on the differentiation programme using the HaCaT keratinocytes model.
methodsHaCaT keratinocytes were incubated with CRH in Dulbecco's modified Eagles's medium (containing 1.8 mmol L(-1) calcium) or EpiLife (containing 0.06 mmol L(-1) calcium) medium. Cell proliferation was assessed with the MTT assay. Flow cytometry was used for the measurement of DNA content, cell size and granularity and the expression of cytokeratin 14, cytokeratin 1 and involucrin. The electrophoretic mobility shift assay was used to determine DNA binding activity by AP-1 transcription factor. Expression of cytokeratin 1 was also assessed with immunofluorescence microscopy.
resultsCRH did produce inhibition of proliferation, which was dose-dependent; the shape of the inhibition curve was determined by the media calcium concentration. CRH action was pinpointed at inhibition of the G0/1 to the S phase transition of the cell cycle. CRH also increased AP-1 binding activity, cell granularity, cytokeratin 1 and involucrin expression, and inhibited cytokeratin 14 expression.
conclusionsThese results are consistent with CRH induction of the keratinocyte differentiation programme. Thus, the overall CRH cutaneous actions connote protective functions for the epidermis, that appear to include the triggering or acceleration of the differentiation programme.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.