ArticleInfection and immunity2005
Involvement of the Escherichia coli O157:H7(pO157) ecf operon and lipid A myristoyl transferase activity in bacterial survival in the bovine gastrointestinal tract and bacterial persistence in farm water troughs.
Article in Infection and immunity, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
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- Metallohelices that kill Gram-negative pathogens using intracellular antimicrobial peptide pathways.Chemical science · 2019Article
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- Disruption of rcsB by a duplicated sequence in a curli-producing Escherichia coli O157:H7 results in differential gene expression in relation to biofilm formation, stress responses and metabolism.BMC microbiology · 2017Article
- Review
- The microcosm mediates the persistence of shiga toxin-producing Escherichia coli in freshwater ecosystems.Applied and environmental microbiology · 2013Article
- Article
- pO157_Sal, a novel conjugative plasmid detected in outbreak isolates of Escherichia coli O157:H7.Journal of clinical microbiology · 2011Article
- Characterization of BNT2, an intrinsically curved DNA of Escherichia coli O157:H7.Biochemical and biophysical research communications · 2010Article
- A brief overview of Escherichia coli O157:H7 and its plasmid O157.Journal of microbiology and biotechnology · 2010Review
- Article
- All blood, no stool: enterohemorrhagic Escherichia coli O157:H7 infection.Journal of veterinary science · 2008Review
- PagP activation in the outer membrane triggers R3 core oligosaccharide truncation in the cytoplasm of Escherichia coli O157:H7.The Journal of biological chemistry · 2008Article
- Sensing gram-negative bacterial lipopolysaccharides: a human disease determinant?Infection and immunity · 2008Review
- Characterization of an Escherichia coli O157:H7 plasmid O157 deletion mutant and its survival and persistence in cattle.Applied and environmental microbiology · 2007Article
- Extensive genomic diversity and selective conservation of virulence-determinants in enterohemorrhagic Escherichia coli strains of O157 and non-O157 serotypes.Genome biology · 2007Article
- Role of Escherichia coli O157:H7 virulence factors in colonization at the bovine terminal rectal mucosa.Infection and immunity · 2006Article
- Shield as signal: lipopolysaccharides and the evolution of immunity to gram-negative bacteria.PLoS pathogens · 2006Review
- Inactivation of enterohemorrhagic Escherichia coli in rumen content- or feces-contaminated drinking water for cattle.Applied and environmental microbiology · 2006Article
- The Escherichia coli O157 flagellar regulatory gene flhC and not the flagellin gene fliC impacts colonization of cattle.Infection and immunity · 2006Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Escherichia coli O157:H7 is an important food-borne pathogen that causes hemorrhagic colitis and the hemolytic-uremic syndrome in humans. Recently, we reported that the pO157 ecf (E. coli attaching and effacing gene-positive conserved fragments) operon is thermoregulated by an intrinsically curved DNA and contains the genes for bacterial surface-associated proteins, including a second copy of lipid A myristoyl transferase, whose chromosomal copy is the lpxM gene product. E. coli O157:H7 survives and persists well in diverse environments from the human and bovine gastrointestinal tracts (GIT) to nutrient-dilute farm water troughs. Transcriptional regulation of the ecf operon by intrinsic DNA curvature and the genetic redundancy of lpxM that is associated with lipid A modification led us to hypothesize that the pO157 ecf operon and lpxM are associated with bacterial survival and persistence in various in vivo and ex vivo environments by optimizing bacterial membrane structure and/or integrity. To test this hypothesis, three isogenic ecf operon and/or lpxM deletion mutants of E. coli O157:H7 ATCC 43894 were constructed and analyzed in vitro and in vivo. The results showed that a double mutant carrying deletions in the ecf and lpxM genes had an altered lipid A structure and membrane fatty acid composition, did not survive passage through the bovine GIT, did not persist well in farm water troughs, had increased susceptibility to a broad spectrum of antibiotics and detergents, and had impaired motility. Electron microscopic analyses showed gross changes in bacterial membrane structure.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.