ArticleInfection and immunity2005
Biofilm Formation by Neisseria gonorrhoeae.
Article in Infection and immunity, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
69 citing papers in PubMed, 112 citations in OpenAlex.
- The role of extracellular DNA inJournal of bacteriology · 2026Review
- An organotypic ectocervix mucosa model to study host-pathogen interaction of sexually transmitted infections.Materials today. Bio · 2026Article
- Vaginal microbiomes and their pertinent social context: a microbial ecological review proffering AMR-STI acquisition and emergence.Biology of sex differences · 2026Review
- Influence of the ATP-dependent DNA ligase, Lig E, onBiofilm · 2025Article
- Gentamicin induction of the gonococcalmBio · 2025Article
- Leveraging the microbiome to combat antibiotic resistant gynecological infections.npj antimicrobials and resistance · 2025Review
- Zap the clap with DNA: a novel microbicide for preventingAntimicrobial agents and chemotherapy · 2024Article
- Cyclization increases bactericidal activity of arginine-rich cationic cell-penetrating peptide forMicrobiology spectrum · 2024Article
- Dinner date: Neisseria gonorrhoeae central carbon metabolism and pathogenesis.Emerging topics in life sciences · 2024Review
- A role for the ATP-dependent DNA ligase lig E of Neisseria gonorrhoeae in biofilm formation.BMC microbiology · 2024Article
- G-quadruplex motifs in Neisseria gonorrhoeae as anti-gonococcal targets.Applied microbiology and biotechnology · 2023Article
- Bacterium of one thousand and one variants: genetic diversity ofMicrobial genomics · 2023Review
- The Gonococcal Genetic Island defines distinct sub-populations ofMicrobial genomics · 2023Article
- Article
- Antibacterial activity of 18β-glycyrrhetinic acid againstBiochemistry and biophysics reports · 2023Article
- In Vitro Anti-Microorganisms · 2023Article
- PotentmSphere · 2022Article
- Assuring the Biofunctionalization of Silicone Covalently Bonded to Rhamnolipids: Antibiofilm Activity and Biocompatibility.Pharmaceutics · 2022Article
- Advances in the Sensing and Treatment of Wound Biofilms.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2022Review
- Advances in the Sensing and Treatment of Wound Biofilms.Angewandte Chemie (International ed. in English) · 2022Review
9 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Studies were performed in continuous-flow chambers to determine whether Neisseria gonorrhoeae could form a biofilm. Under these growth conditions, N. gonorrhoeae formed a biofilm with or without the addition of 10 microM sodium nitrite to the perfusion medium. Microscopic analysis of a 4-day growth of N. gonorrhoeae strain 1291 revealed evidence of a biofilm with organisms embedded in matrix, which was interlaced with water channels. N. gonorrhoeae strains MS11 and FA1090 were found to also form biofilms under the same growth conditions. Cryofield emission scanning electron microscopy and transmission electron microscopy confirmed that organisms were embedded in a continuous matrix with membranous structures spanning the biofilm. These studies also demonstrated that N. gonorrhoeae has the capability to form a matrix in the presence and absence of CMP-N-acetylneuraminic acid (CMP-Neu5Ac). Studies with monoclonal antibody 6B4 and the lectins soy bean agglutinin and Maackia amurensis indicated that the predominate terminal sugars in the biofilm matrix formed a lactosamine when the biofilm was grown in the absence of CMP-Neu5Ac and sialyllactosamine in the presence of CMP-Neu5Ac. N. gonorrhoeae strain 1291 formed a biofilm on primary urethral epithelial cells and cervical cells in culture without loss of viability of the epithelial cell layer. Our studies demonstrated that N. gonorrhoeae can form biofilms in continuous-flow chambers and on living cells. Studies of these biofilms may have implications for understanding asymptomatic gonococcal infection.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.